Evidence map›Paper›PMID 8600839›Full record

ArticleAnalytical biochemistry1995

A lifetime-based fluorescence resonance energy transfer sensor for ammonia.

Q Chang, J Sipior, J R Lakowicz, G Rao

Abstract read
In one paragraph

Article in Analytical biochemistry, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Long-lifetime metal-ligand pH probe.Analytical biochemistry · 1997
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Q ChangDepartment of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Maryland 21227, USA.
J Sipior
J R Lakowicz
G Rao

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI CHEUNG, HERBERT C · 1993 to 2006
$7.4M
ARGON LASER FOR TIME-AND FREQUENCY-DOMAIN FLUORESCENCES10RR007510 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 1991 to 1991
–
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-07510NCRR NIH HHS RR-08119
6 · The paper itself

Abstract

A lifetime-based optical NH3 sensor based on the principle of fluorescence resonance energy transfer was developed. The sensor consisted of sulforhodamine 101 as the donor, bromocresol green as the acceptor, ethyl cellulose as the polymer support, and tributyl phosphate as the plasticizer. When the concentration of NH3 changed, it caused a change in the decay time of the SR101, which was measured by phase-modulation fluorometry. At 100 MHz, increasing the concentration of NH3 from 0 to 175 ppm resulted in a decrease in phase angle of about 31 degrees and an increase in modulation of about 18%. Oxygen and carbon dioxide did not interfere with the sensor. However, a 30% relative humidity could cause a downward shift of the response by 5 degrees, while additional increase in the relative humidity to 100% showed little further effect. For a film thickness of 40 microns, the typical response and recovery times for 90% of total signal change were 1 and 2.5 min, respectively. The phase angle measurements for the same sample were reproducible for 5 days, with no special care of the film sample.

Indexed as

Biosensing TechniquesAmmoniaBromcresol GreenCalibrationCelluloseEnergy TransferHumidityOptics and PhotonicsReproducibility of ResultsRhodaminesSpectrometry, FluorescenceAmmoniaBromcresol GreenCelluloseethyl celluloseRhodaminessulforhodamine 101

Identifiers

PMID8600839
PMCPMC6905383

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.