ArticleThe EMBO journal1996
A two-step recognition of signal sequences determines the translocation efficiency of proteins.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 96 citations in OpenAlex.
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- GPR18 undergoes a high degree of constitutive trafficking but is unresponsive to N-Arachidonoyl Glycine.PeerJ · 2016Article
- The putative signal peptide of glucagon-like peptide-1 receptor is not required for receptor synthesis but promotes receptor expression.Bioscience reports · 2014Article
- Identification of steroid-sensitive gene-1/Ccdc80 as a JAK2-binding protein.Molecular endocrinology (Baltimore, Md.) · 2013Article
- SGTA antagonizes BAG6-mediated protein triage.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Chloroplast envelope protein targeting fidelity is independent of cytosolic components in dual organelle assays.Frontiers in plant science · 2012Article
- The extended signal peptide of the trimeric autotransporter EmaA of Aggregatibacter actinomycetemcomitans modulates secretion.Journal of bacteriology · 2011Article
- Controlling subcellular delivery to optimize therapeutic effect.Therapeutic delivery · 2010Review
- Signal sequence insufficiency contributes to neurodegeneration caused by transmembrane prion protein.The Journal of cell biology · 2010Article
- Cellular mechanisms of membrane protein folding.Nature structural & molecular biology · 2009Article
- Identification of a post-targeting step required for efficient cotranslational translocation of proteins across the Escherichia coli inner membrane.The Journal of biological chemistry · 2009Article
- Calnuc plays a role in dynamic distribution of Galphai but not Gbeta subunits and modulates ACTH secretion in AtT-20 neuroendocrine secretory cells.Molecular neurodegeneration · 2009Article
- Control of translocation through the Sec61 translocon by nascent polypeptide structure within the ribosome.The Journal of biological chemistry · 2008Article
- Alu RNP and Alu RNA regulate translation initiation in vitro.Nucleic acids research · 2006Article
- N-myristoylation determines dual targeting of mammalian NADH-cytochrome b5 reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning.The Journal of cell biology · 2005Article
- An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Biogenesis of CFTR and other polytopic membrane proteins: new roles for the ribosome-translocon complex.The Journal of membrane biology · 2004Review
- Cytosolic prion protein in neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Bimodal targeting of microsomal CYP2E1 to mitochondria through activation of an N-terminal chimeric signal by cAMP-mediated phosphorylation.The Journal of biological chemistry · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytosolic and secreted, N-glycosylated, forms of plasminogen activator inhibitor-2 (PAI-2) are generated by facultative translocation. To study the molecular events that result in the bi-topological distribution of proteins, we determined in vitro the capacities of several signal sequences to bind the signal recognition particle (SRP) during targeting, and to promote vectorial transport of murine PAI-2 (mPAI-2). Interestingly, the six signal sequences we compared (mPAI-2 and three mutated derivatives thereof, ovalbumin and preprolactin) were found to have the differential activities in the two events. For example, the mPAI-2 signal sequence first binds SRP with moderate efficiency and secondly promotes the vectorial transport of only a fraction of the SRP-bound nascent chains. Our results provide evidence that the translocation efficiency of proteins can be controlled by the recognition of their signal sequences at two steps: during SRP-mediated targeting and during formation of a committed translocation complex. This second recognition may occur at several time points during the insertion/translocation step. In conclusion, signal sequences have a more complex structure than previously anticipated, allowing for multiple and independent interactions with the translocation machinery.
Indexed as
Identifiers
8599930PMC449965W163282153What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.