ArticleBiophysical journal1995
Selective adhesion of functional microtubules to patterned silane surfaces.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 107 citations in OpenAlex.
- Sensing surface mechanical deformation using active probes driven by motor proteins.Nature communications · 2016Article
- Application of 2D Non-Graphene Materials and 2D Oxide Nanostructures for Biosensing Technology.Sensors (Basel, Switzerland) · 2016Review
- Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Translational actomyosin research: fundamental insights and applications hand in hand.Journal of muscle research and cell motility · 2012Review
- Optical biosensors: a revolution towards quantum nanoscale electronics device fabrication.Journal of biomedicine & biotechnology · 2011Review
- Resolving the molecular structure of microtubules under physiological conditions with scanning force microscopy.European biophysics journal : EBJ · 2004Article
- Shear flow-induced detachment kinetics of Dictyostelium discoideum cells from solid substrate.Biophysical journal · 2002Article
- Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks.Biophysical journal · 2001Article
- Actin motion on microlithographically functionalized myosin surfaces and tracks.Biophysical journal · 1999Article
- Control of actin moving trajectory by patterned poly(methylmethacrylate) tracks.Biophysical journal · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that microtubule polymers can be immobilized selectively on lithographically patterned silane surfaces while retaining their native properties. Silane films were chemisorbed on polished silicon wafers or glass coverslips and patterned using a deep UV lithographic process developed at the Naval Research Laboratory. Hydrocarbon and fluorocarbon alkyl silanes, as well as amino and thiol terminal alkyl silanes, were investigated as substrates for microtubule adhesion with retention of biological activity. Microtubules were found to adhere strongly to amine terminal silanes while retaining the ability to act as substrates for the molecular motor protein kinesin. Aminosilane patterns with linewidths varying from 1 to 50 microns were produced lithographically and used to produce patterns of selectively adhered microtubules. Microtubules were partially aligned on the patterned lines by performing the immobilization in a fluid flow field. Patterns were imaged with atomic force microscopy and differential interference contrast microscopy. Motility assays were carried out using kinesin-coated beads and observed with differential interference contrast microscopy. Kinesin bead movement on the patterned microtubules was comparable to movement on microtubule control surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.