ArticleBiophysical journal1995
Fluorescence lifetime imaging by asynchronous pump-probe microscopy.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 112 citations in OpenAlex.
- Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.APL photonics · 2026Article
- Nonlinear optical microscopy with an obscuration-free, freeform reflective objective.Biomedical optics express · 2025Article
- Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023Article
- Progress and Prospects in Optical Ultrafast Microscopy in the Visible Spectral Region: Transient Absorption and Two-Dimensional Microscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023Review
- Fluorescence-Detected Pump-Probe Spectroscopy.Angewandte Chemie (International ed. in English) · 2021Article
- Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy.Frontiers in bioinformatics · 2021Article
- Transient absorption microscopy: Technological innovations and applications in materials science and life science.The Journal of chemical physics · 2020Article
- Label-Free Imaging of Heme Dynamics in Living Organisms by Transient Absorption Microscopy.Analytical chemistry · 2018Article
- Invited Review Article: Pump-probe microscopy.The Review of scientific instruments · 2016Review
- Coherent nonlinear optical imaging: beyond fluorescence microscopy.Annual review of physical chemistry · 2011Review
- Article
- One photon up, one photon down.Nature biotechnology · 2009Article
- Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis.Biophysical journal · 2005Article
- Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence microscopy.Biophysical journal · 2000Article
- Global analysis of fluorescence lifetime imaging microscopy data.Biophysical journal · 2000Article
- Lateral resolution enhancement with standing evanescent waves.Optics letters · 2000Article
- Lifetime-based pH sensors: indicators for acidic environments.Analytical biochemistry · 1999Article
- Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin.Biophysical journal · 1997Article
- Light quenching of pyridine2 fluorescence with time-delayed pulses.Biophysical chemistry · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We report the development of a scanning lifetime fluorescence microscope using the asynchronous, pump-probe (stimulated emission) approach. There are two significant advantages of this technique. First, the cross-correlation signal produced by overlapping the pump and probe lasers results in i) an axial sectioning effect similar to that in confocal and two-photon excitation microscopy, and ii) improved spatial resolution compared to conventional one-photon fluorescence microscopy. Second, the low-frequency, cross-correlation signal generated allows lifetime-resolved imaging without using fast photodetectors. The data presented here include 1) determination of laser sources' threshold powers for linearity in the pump-probe signal; 2) characterization of the pump-probe intensity profile using 0.28 microns fluorescent latex spheres; 3) high frequency (up to 6.7 GHz) lifetime measurement of rhodamine B in water; and 4) lifetime-resolved images of fluorescent latex spheres, human erythrocytes and a mouse fibroblast cell stained by rhodamine DHPE, and a mouse fibroblast labeled with ethidium bromide and rhodamine DHPE.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.