ArticleThe EMBO journal1996
GPI anchor attachment is required for Gas1p transport from the endoplasmic reticulum in COP II vesicles.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed.
- GPI-anchored Gas1 protein regulates cytosolic proteostasis in budding yeast.G3 (Bethesda, Md.) · 2024Article
- Structure and Function of the Glycosylphosphatidylinositol Transamidase, a Transmembrane Complex Catalyzing GPI Anchoring of Proteins.Sub-cellular biochemistry · 2024Review
- GPI-anchored Gas1 protein regulates cytosolic proteostasis in yeast.bioRxiv : the preprint server for biology · 2023Article
- A defect in GPI synthesis as a suggested mechanism for the role of ARV1 in intellectual disability and seizures.Neurogenetics · 2020Article
- Impaired GCR1 transcription resulted in defective inositol levels, vacuolar structure and autophagy in Saccharomyces cerevisiae.Current genetics · 2019Article
- Article
- Controlling transferrin receptor trafficking with GPI-valence in bloodstream stage African trypanosomes.PLoS pathogens · 2017Article
- FERONIA and Her Pals: Functions and Mechanisms.Plant physiology · 2016Article
- The Role of LORELEI in Pollen Tube Reception at the Interface of the Synergid Cell and Pollen Tube Requires the Modified Eight-Cysteine Motif and the Receptor-Like Kinase FERONIA.The Plant cell · 2016Article
- The response to inositol: regulation of glycerolipid metabolism and stress response signaling in yeast.Chemistry and physics of lipids · 2014Review
- Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter.Biotechnology for biofuels · 2014Article
- Review
- Defining the boundaries of species specificity for the Saccharomyces cerevisiae glycosylphosphatidylinositol transamidase using a quantitative in vivo assay.Bioscience reports · 2012Article
- The membrane stress response buffers lethal effects of lipid disequilibrium by reprogramming the protein homeostasis network.Molecular cell · 2012Article
- Stress-induced synthesis of phosphatidylinositol 3-phosphate in mycobacteria.The Journal of biological chemistry · 2010Article
- Requirements for transitional endoplasmic reticulum site structure and function in Saccharomyces cerevisiae.Molecular biology of the cell · 2010Article
- Convergent extension movements in growth plate chondrocytes require gpi-anchored cell surface proteins.Development (Cambridge, England) · 2009Article
- Molecular biology, genetics and biochemistry of the repulsive guidance molecule family.The Biochemical journal · 2009Review
- Strategies for acquiring the phospholipid metabolite inositol in pathogenic bacteria, fungi and protozoa: making it and taking it.Microbiology (Reading, England) · 2009Review
- Role of Sec61p in the ER-associated degradation of short-lived transmembrane proteins.The Journal of cell biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inositol starvation of auxotrophic yeast interrupts glycolipid biosynthesis and prevents lipid modification of a normally glycosyl phosphatidylinositol (GPI)-linked protein, Gas1p. The unanchored Gas1p precursor undergoes progressive modification in the endoplasmic reticulum (ER), but is not modified by Golgi-specific glycosylation. Starvation-induced defects in anchor assembly and protein processing are rapid, and occur without altered maturation of other proteins. Cells remain competent to manufacture anchor components and to process Gas1p efficiently once inositol is restored. Newly synthesized Gas1p is packaged into vesicles formed in vitro from perforated yeast spheroplasts incubated with either yeast cytosol or the purified Sec proteins (COP II) required for vesicle budding from the ER. In vitro synthesized vesicles produced by inositol-starved membranes do not contain detectable Gas1p. These studies demonstrate that COP II components fulfill the soluble protein requirements for packaging a GPI-anchored protein into ER-derived transport vesicles. However, GPI anchor attachment is required for this packaging to occur.
Indexed as
Identifiers
8598201PMC449930What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.