ArticlePflugers Archiv : European journal of physiology1996
Further investigation of ionic diffusive properties and of NH4+ pathways in Xenopus laevis oocyte cell membrane.
Article in Pflugers Archiv : European journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 42 citations in OpenAlex.
- Aquaporin 4 as a NH3 Channel.The Journal of biological chemistry · 2016Article
- Antennal-expressed ammonium transporters in the malaria vector mosquito Anopheles gambiae.PloS one · 2014Article
- Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S.American journal of physiology. Cell physiology · 2011Article
- Sodium-bicarbonate cotransporter NBCn1/Slc4a7 inhibits NH4Cl-mediated inward current in Xenopus oocytes.Experimental physiology · 2011Article
- pH sensitivity of ammonium transport by Rhbg.American journal of physiology. Cell physiology · 2010Article
- Calcium dynamics during physiological acidification in Xenopus oocyte.The Journal of membrane biology · 2010Article
- The actual ionic nature of the leak current through the Na+/glucose cotransporter SGLT1.Biophysical journal · 2010Article
- Test of blockers of AQP1 water permeability by a high-resolution method: no effects of tetraethylammonium ions or acetazolamide.Pflugers Archiv : European journal of physiology · 2008Article
- Different transport mechanisms in plant and human AMT/Rh-type ammonium transporters.The Journal of general physiology · 2006Article
- Human Rhesus B and Rhesus C glycoproteins: properties of facilitated ammonium transport in recombinant kidney cells.The Biochemical journal · 2005Article
- NH3 and NH4+ permeability in aquaporin-expressing Xenopus oocytes.Pflugers Archiv : European journal of physiology · 2005Article
- Electroneutral ammonium transport by basolateral rhesus B glycoprotein.The Journal of physiology · 2004Article
- Glucose accumulation can account for the initial water flux triggered by Na+/glucose cotransport.Biophysical journal · 2004Article
- NH(4)(+) conductance in Xenopus laevis oocytes. III. Effect of NH(3).Pflugers Archiv : European journal of physiology · 2003Article
- Transport of volatile solutes through AQP1.The Journal of physiology · 2002Review
- Does the colonic H,K-ATPase also act as an Na,K-ATPase?Proceedings of the National Academy of Sciences of the United States of America · 1998Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the ionic diffusive properties and the NH4+ pathways in the Xenopus laevis oocyte cell membrane, we recorded the effects of various inhibitors on membrane potential (Vm) and membrane resistance (Rm); intracellular acidification was taken as an index of NH4+ influx from the bath to the cytoplasm. The following results were obtained: in the control state, barium and quinine (Q) ions depolarized Vm and raised Rm, consistent with inhibition of K+ conductance(s). Diphenylamine-2-carboxylic acid (DPC), 3',5'-dichlorodiphenylamine-2-carboxylic acid (DCDPC) and gadolinium ions hyperpolarized Vm and increased Rm, suggesting the inhibition of nonselective cationic conductance(s). In the presence of 20 mmol/l NH4Cl, Vm depolarized, Rm fell, and intracellular pH (pHi) decreased, consistent with an NH4+ influx. In the presence of DPC, the same manoeuvre induced a biphasic Vm change (i.e. a spike depolarization followed by a membrane hyperpolarization) and a fall of Rm; in most oocytes, intracellular acidification persisted and was reversible upon adding ouabain (Oua). These results indicate that a DPC-sensitive conductance is not the unique NH4+ pathway and that Na, K-ATPase may also mediate NH4+ influx. However, Oua did not prevent the Rm decrease, suggesting that ouabain-insensitive rheogenic pathway(s) are activated. Thus, we investigated the Vm change induced by NH4Cl addition in the presence of DPC: the spike depolarization followed by secondary hyperpolarization became a plateau depolarization when Q was added, suggesting involvement of Q-sensitive pathway(s) in the above described biphasic Vm change. In the presence of DPC, Q and Oua, intracellular acidification upon adding NH4Cl persisted consistent with further NH4+ influx through quinine-, DPC- and Oua-insensitive pathway(s).
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