ArticleGlycoconjugate journal1995
Sialic acid-binding lectins: submolecular specificity and interaction with sialoglycoproteins and tumour cells.
Article in Glycoconjugate journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- LeGenD: High-throughput N-glycan profiling using explainable AI and lectin profiling.The Journal of biological chemistry · 2026Article
- CRISPR activation screens map the genomic landscape of cancer glycome remodeling.Cell genomics · 2026Article
- LeGenD: determining N-glycoprofiles using an explainable AI-leveraged model with lectin profiling.bioRxiv : the preprint server for biology · 2024Article
- Tumor identificationChemical science · 2023Article
- Recent Advances in Lectin-Based Affinity Sorbents for Protein Glycosylation Studies.Frontiers in molecular biosciences · 2021Review
- Altered Cell Surface N-Glycosylation of Resting and Activated T Cells in Systemic Lupus Erythematosus.International journal of molecular sciences · 2019Article
- Quantification of lectin fluorescence in GNE myopathy muscle biopsies.Muscle & nerve · 2018Article
- Studying the effects of reproductive hormones and bacterial vaginosis on the glycome of lavage samples from the cervicovaginal cavity.PloS one · 2015Article
- Determination of major sialylated N-glycans and identification of branched sialylated N-glycans that dynamically change their content during development in the mouse cerebral cortex.Glycoconjugate journal · 2014Article
- Comparative studies of the proteome, glycoproteome, and N-glycome of clear cell renal cell carcinoma plasma before and after curative nephrectomy.Journal of proteome research · 2014Article
- Partial enzymatic deglycosylation preserves the structure of cleaved recombinant HIV-1 envelope glycoprotein trimers.The Journal of biological chemistry · 2012Article
- Comparison of lectin binding of drusen, RPE, Bruch's membrane, and photoreceptors.Molecular vision · 2009Article
- Specificity of the binding site of the sialic acid-binding lectin from ovine placenta, deduced from interactions with synthetic analogues.Glycoconjugate journal · 2000Article
- Cepaea hortensis agglutinin-I, specific for O-glycosidically linked sialic acids, selectively labels endothelial cells of distinct vascular beds.The Histochemical journal · 2000Article
- Lectin analysis of human immunoglobulin G N-glycan sialylation.Glycoconjugate journal · 1999Article
- Identification of CD22 ligands on bone marrow sinusoidal endothelium implicated in CD22-dependent homing of recirculating B cells.The Journal of experimental medicine · 1999Article
- A comparative study of lectin binding to cultured chick sternal chondrocytes and intact chick sternum.Glycoconjugate journal · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the specificity of limulin, Limax flavus agglutinin (LFA) and Sambucus nigra agglutinin I (SNA I) at the submolecular level of sialic acid, and characterized their interactions with a panel of structurally distinct sialoglycoproteins. In haemagglutination inhibition assays NeuAc-alpha-glycosides were stronger inhibitors for limulin and LFA than native N-acetylneuraminic acid (NeuAc). The N-acetyl of NeuAc was crucial for binding to both lectins. N-thioacetylated NeuAc lost affinity for LFA, but still bound to limulin. Thus, distinct intermolecular interactions are involved in binding of sialic acid to the lectins. The glyceryl side chain was required for interaction with LFA, but not with limulin. SNA I specifically bound NeuAc alpha 2 --> 6Gal beta 1 --> 4Glc, but not monomeric sialic acids. Limulin and LFA strongly interacted with O-chain glycoproteins, whereas SNA I preferred N-chain proteins that carry NeuAc alpha 2 --> 6 residues. The lectins were compared with those from Cepaea hortensis and Tachypleus tridentatus (TTA) and to wheat-germ agglutinin, and were then used to probe tumour cell lines for cell surface sialylation. With the exception of TTA, all lectins interacted with the tumour cells. Limulin distinguished between the low (Eb) and highly (ESb) metastatic mouse lymphoma lines by selectively agglutinating sialidase-treated ESb cells.
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