ArticleCurrent genetics1995
Characterization of the mitochondrial orfB gene and its derivative, orf224, a chimeric open reading frame specific to one mitochondrial genome of the "Polima" male-sterile cytoplasm in rapeseed (Brassica napus L.).
Article in Current genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Energy Metabolism and Auxin Signaling Disruption Underlying Stamen Identity Defects in Tobacco Cytoplasmic Male Sterility K326 (CMS K326): Integrated Transcriptomic and Metabolomic Analyses.Plants (Basel, Switzerland) · 2026Article
- Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Integrated Methylome and Transcriptome Analysis Widen the Knowledge of Cytoplasmic Male Sterility in Cotton (Frontiers in plant science · 2022Article
- Organelle Comparative Genome Analysis Reveals Novel Alloplasmic Male Sterility withInternational journal of molecular sciences · 2021Article
- Molecular Analysis Uncovers the Mechanism of Fertility Restoration in Temperature-Sensitive Polima Cytoplasmic Male-SterileInternational journal of molecular sciences · 2021Article
- High-generation near-isogenic lines combined with multi-omics to study the mechanism of polima cytoplasmic male sterility.BMC plant biology · 2021Article
- Elucidating Mitochondrial DNA Markers ofFrontiers in plant science · 2021Article
- Comparison of the cytoplastic genomes by resequencing: insights into the genetic diversity and the phylogeny of the agriculturally important genus Brassica.BMC genomics · 2020Article
- Comparative Transcriptome Analysis between a Novel Allohexaploid Cotton Progeny CMS Line LD6A and Its Maintainer Line LD6B.International journal of molecular sciences · 2019Article
- Organelle genome composition and candidate gene identification for Nsa cytoplasmic male sterility in Brassica napus.BMC genomics · 2019Article
- Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding.Plant reproduction · 2019Review
- Molecular Approaches for Manipulating Male Sterility and Strategies for Fertility Restoration in Plants.Molecular biotechnology · 2017Review
- The application of RNA-seq to the comprehensive analysis of plant mitochondrial transcriptomes.Molecular genetics and genomics : MGG · 2015Review
- Article
- Isolation and characterization of a male sterility gene homolog BcMS2 from Chinese cabbage-pak-choi that expressing in an anther-specific manner.Molecular biology reports · 2008Article
- Development of a molecular marker specific to a novel CMS line in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Interactions of mitochondrial and nuclear genes that affect male gametophyte development.The Plant cell · 2004Review
- The complete nucleotide sequence and RNA editing content of the mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis of the mitochondrial genomes of rapeseed and Arabidopsis thaliana.Nucleic acids research · 2003Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
orf224 is a novel reading frame present upstream of the atp6 gene in the mitochondria of "Polima" cms cytoplasm of rapeseed. In order to determine the origin of orf224, the sequences homologous to orf224 were isolated and characterized. Sequence analysis indicated that orf224 originated by recombination events involving the 5'-flanking region and the amino-terminal segment of the coding region of orf158 (well-known as orfB in other plants), part of exon 1 of the ribosomal protein S3 (rps3) gene, and an unidentified sequence. Transcripts of the orf158 gene were found to be edited at three positions, one of which induces an amino-acid change, while orf224 transcripts have only one RNA editing site within the region homologous to the rps3 gene. This editing site is also present in the proper rps3 transcripts. This result indicates that editing of orf224 occurred because of the sequence homology to rps3. Polyclonal antibodies prepared against a rapeseed ORF158 fusion protein specifically recognize a 18-kDa protein in the membrane fractions of mitochondria from both normal and cms rapeseed.
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