ArticleMolecular biology of the cell1995
Nuclear and nucleolar targeting of human ribosomal protein S6.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Integrative Bioinformatics Analysis of The Cell Division Cycle and Ribosomal Pathways in The Rat Varicocele: Implications for Drug Discovery.Cell journal · 2023Article
- Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma.Oncology reports · 2023Article
- Ribosomal Protein S6: A Potential Therapeutic Target against Cancer?International journal of molecular sciences · 2021Review
- Article
- Interplay between human nucleolar GNL1 and RPS20 is critical to modulate cell proliferation.Scientific reports · 2018Article
- Mass Spectrometry Based Proteomics Study of Cisplatin-Induced DNA-Protein Cross-Linking in Human Fibrosarcoma (HT1080) Cells.Chemical research in toxicology · 2017Article
- Identification of cargo proteins specific for the nucleocytoplasmic transport carrier transportin by combination of an in vitro transport system and stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics.Molecular & cellular proteomics : MCP · 2013Article
- Elucidation of motifs in ribosomal protein S9 that mediate its nucleolar localization and binding to NPM1/nucleophosmin.PloS one · 2012Article
- Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus.Journal of virology · 2011Article
- Clusters of basic amino acids contribute to RNA binding and nucleolar localization of ribosomal protein L22.PloS one · 2009Article
- Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis.BMC cell biology · 2008Article
- Subcellular localization of CIAPIN1.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2006Article
- Ribosomal protein S6 associates with alphavirus nonstructural protein 2 and mediates expression from alphavirus messages.Journal of virology · 2006Article
- Article
- Dimerization of MLH1 and PMS2 limits nuclear localization of MutLalpha.Molecular and cellular biology · 2003Article
- Functional characterization of nuclear localization signals in yeast Sm proteins.Molecular and cellular biology · 2000Article
- Cooperative signals governing ARF-mdm2 interaction and nucleolar localization of the complex.Molecular and cellular biology · 2000Article
- Constitutively active MuSK is clustered in the absence of agrin and induces ectopic postsynaptic-like membranes in skeletal muscle fibers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Nuclear and nucleolar targeting of human ribosomal protein S25: common features shared with HIV-1 regulatory proteins.Oncogene · 1999Article
- Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chimeric proteins were constructed to define the nuclear localization signals (NLSs) of human ribosomal protein S6. The complete cDNA sequence, different cDNA fragments and oligonucleotides of the human ribosomal proteins S6, respectively, were joined to the 5' end of the entire LacZ gene of Escherichia coli by using recombinant techniques. The hybrid genes were transfected into L cells, transiently expressed, and the intracellular location of the fusion proteins was determined by their beta-galactosidase activity. Three NLSs were identified in the C-terminal half of the S6 protein. Deletion mutagenesis demonstrated that a single NLS is sufficient for targeting the corresponding S6-beta-galactosidase chimera into the nucleus. Removal of all three putative NLSs completely blocked the nuclear import of the resulting S6-beta-galactosidase fusion protein, which instead became evenly distributed in the cytoplasm. Chimeras containing deletion mutants of S6 with at least one single NLS or unmodified S6 accumulated in the nucleolus. Analysis of several constructs reveals the existence of a specific domain that is essential but not sufficient for nucleolar accumulation of S6.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.