ArticleMolecular biology of the cell1995
Cell adhesion to extracellular matrix regulates the life cycle of integrins.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 72 citations in OpenAlex.
- Sweet and sticky: increased cell adhesion through click-mediated functionalization of regenerative liver progenitor cells.Communications biology · 2025Article
- Machine Learning Classification of Integrin-Expression-Based Magnetic Sorted SW 620 Cells by Simultaneous O-PTIR and SERS.Analytical chemistry · 2024Article
- Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes.Frontiers in chemistry · 2019Article
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- The roles of ADAMTS in angiogenesis and cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Review
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- Neural tissue continues its maturation at the site of neural tube closure defects: implications for prenatal intervention in human samples.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2004Article
- Sulphatides trigger polymorphonuclear granulocyte spreading on collagen-coated surfaces and inhibit subsequent activation of 5-lipoxygenase.The Biochemical journal · 2001Article
- Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells.The Journal of cell biology · 2001Article
- Unique expression pattern of the alpha6beta4 integrin and laminin-5 in human prostate carcinoma.The Prostate · 2001Article
- Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK.The EMBO journal · 2000Article
- Signaling via beta1 integrins and mitogen-activated protein kinase determines human epidermal stem cell fate in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Integrins (alpha7beta1) in muscle function and survival. Disrupted expression in merosin-deficient congenital muscular dystrophy.The Journal of clinical investigation · 1997Article
- Integrin expression regulates neuroblastoma attachment and migration.Neoplasia (New York, N.Y.)Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The expression of alpha 5 beta 1 integrin on the surface of fibroblasts requires adhesion to substratum. We have examined the basis for this adhesion-dependent surface expression by comparing the life cycle of integrins in parallel cultures of adherent and nonadherent cells. Results of biosynthetic labeling experiments in NRK fibroblasts showed that the synthesis and biosynthetic processing of the beta 1 integrin subunit proceed in the absence of cell attachment; however, when examining the behavior of preexisting cell surface integrins, we observed that the alpha beta 1 integrins are internalized and degraded when adhesion to substratum is blocked. A kinetic analysis of integrin internalization in cycloheximide-treated NRK cells showed that each of the fibroblast integrins we examined (in both the beta 1 and beta 3 families) are lost from the cell surface after detachment from substratum. Thus, the default integrin life cycle in fibroblasts involves continuous synthesis, processing, transport to the cell surface, and internalization/degradation. Interestingly, studies with NIH-3T3 cells expressing alpha 1 beta 1 integrin showed that the loss of cell-surface alpha 5 beta 1 integrin is blocked by adhesion of cells to dishes coated with type IV collagen (a ligand for alpha 1 beta 1 integrin) as well as fibronectin. Similarly, adhesion of these cells to dishes coated with type IV collagen stabilizes the surface expression of alpha 5 beta 1 as well as alpha 1 beta 1 integrin. We propose that the adhesion of fibroblasts to extracellular matrix protein alters the integrin life cycle and permits retention of these proteins at the cell surface where they can play important roles in transmitting adhesion-dependent signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.