ArticleMolecular biology of the cell1995
end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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Who cites it
144 citing papers in PubMed, 301 citations in OpenAlex.
- Pleiotropy drives evolutionary repair of the responsiveness of polarized cell growth to environmental cues.Frontiers in microbiology · 2023Article
- Regulation of membrane scission in yeast endocytosis.Molecular biology of the cell · 2022Article
- ER-phagy requires the assembly of actin at sites of contact between the cortical ER and endocytic pits.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Ist2 recruits the lipid transporters Osh6/7 to ER-PM contacts to maintain phospholipid metabolism.The Journal of cell biology · 2021Article
- Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane.Molecular biology of the cell · 2021Article
- Review
- WHI-2 Regulates Intercellular Communication via a MAP Kinase Signaling Complex.Frontiers in microbiology · 2019Article
- BAR domain proteins-a linkage between cellular membranes, signaling pathways, and the actin cytoskeleton.Biophysical reviews · 2018Review
- The pleiotropic effects of the glutamate dehydrogenase (GDH) pathway in Saccharomyces cerevisiae.Microbial cell factories · 2018Review
- Global Deletome Profile ofFrontiers in microbiology · 2018Article
- The SAGA complex, together with transcription factors and the endocytic protein Rvs167p, coordinates the reprofiling of gene expression in response to changes in sterol composition inMolecular biology of the cell · 2017Article
- Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis.The Journal of cell biology · 2017Article
- MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae.Scientific reports · 2017Article
- Cfs1p, a Novel Membrane Protein in the PQ-Loop Family, Is Involved in Phospholipid Flippase Functions in Yeast.G3 (Bethesda, Md.) · 2017Article
- Fscn1 is required for the trafficking of TGF-β family type I receptors during endoderm formation.Nature communications · 2016Article
- Calmodulin Promotes N-BAR Domain-Mediated Membrane Constriction and Endocytosis.Developmental cell · 2016Article
- An Amphiphysin-Like Domain in Fus2p Is Required for Rvs161p Interaction and Cortical Localization.G3 (Bethesda, Md.) · 2015Article
- Effects of FSGS-associated mutations on the stability and function of myosin-1 in fission yeast.Disease models & mechanisms · 2015Article
- Plasma Membrane Proteolipid 3 Protein Modulates Amphotericin B Resistance through Sphingolipid Biosynthetic Pathway.Scientific reports · 2015Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
84 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four mutants defective in endocytosis were isolated by screening a collection of temperature-sensitive yeast mutants. Three mutations define new END genes: end5-1, end6-1, and end7-1. The fourth mutation is in END4, a gene identified previously. The end5-1, end6-1, and end7-1 mutations do not affect vacuolar protein localization, indicating that the defect in each mutant is specific for internalization at the plasma membrane. Interestingly, localization of actin patches on the plasma membrane is affected in each of the mutants. end5-1, end6-1, and end7-1 are allelic to VRP1, RVS161, and ACT1, respectively. VRP1 and RVS161 are required for correct actin localization and ACT1 encodes actin. To our surprise, the end6-1 mutation fails to complement the act1-1 mutation. Disruption of the RVS167 gene, which is homologous to END6/RVS161 and which is also required for correct actin localization, also blocks endocytosis. The end7-1 mutant allele has a glycine 48 to aspartic acid substitution in the DNase I-binding loop of actin. We propose that Vrp1p, Rvs161p, and Rvs167p are components of a cytoskeletal structure that contains actin and fimbrin and that is required for formation of endocytic vesicles at the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.