ArticleMolecular biology of the cell1995
Gi2-mediated activation of the MAP kinase cascade.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 77 citations in OpenAlex.
- Going Rogue: Mechanisms, Regulation, and Roles of Mutationally Activated GMolecular pharmacology · 2024Review
- Co-receptor signaling in the pathogenesis of neuroHIV.Retrovirology · 2021Review
- The relation between mitogen activated protein kinase (MAPK) pathway and different genes expression in patients with beta Thalassemia.Biochemistry and biophysics reports · 2020Article
- Hepatic Gi signaling regulates whole-body glucose homeostasis.The Journal of clinical investigation · 2018Article
- Pertussis toxin-sensitive signaling of melanocortin-4 receptors in hypothalamic GT1-7 cells defines agouti-related protein as a biased agonist.The Journal of biological chemistry · 2009Article
- GNAI2 and regulators of G protein signaling as a potential Noonan syndrome mechanism.Medical hypotheses · 2009Article
- Article
- S1P1 receptor localization confers selectivity for Gi-mediated cAMP and contractile responses.The Journal of biological chemistry · 2008Article
- Galpha(i2) inhibition of adenylate cyclase regulates presynaptic activity and unmasks cGMP-dependent long-term depression at Schaffer collateral-CA1 hippocampal synapses.Learning & memory (Cold Spring Harbor, N.Y.) · 2008Article
- G protein regulation of MAPK networks.Oncogene · 2007Review
- Pleiotropic phenotype of a genomic knock-in of an RGS-insensitive G184S Gnai2 allele.Molecular and cellular biology · 2006Article
- mGluR2 acts through inhibitory Galpha subunits to regulate transmission and long-term plasticity at hippocampal mossy fiber-CA3 synapses.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Insulin induces heterologous desensitization of G-protein-coupled receptor and insulin-like growth factor I signaling by downregulating beta-arrestin-1.Molecular and cellular biology · 2002Article
- Differential coupling of 5-HT(1) receptors to G proteins of the G(i) family.British journal of pharmacology · 2002Article
- Characterization of subcellular localization and stability of a splice variant of G alpha i2.BMC cell biology · 2002Article
- Evidence for the involvement of Gi2 in activation of extracellular signal-regulated kinases in hepatocytes.BMC cell biology · 2001Article
- Activation of B-Raf and regulation of the mitogen-activated protein kinase pathway by the G(o) alpha chain.Molecular biology of the cell · 2000Article
- Activation of extracellular signal-regulated protein kinases is associated with a sensitized locomotor response to D(2) dopamine receptor stimulation in unilateral 6-hydroxydopamine-lesioned rats.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Mitogenic signaling via endogenous kappa-opioid receptors in C6 glioma cells: evidence for the involvement of protein kinase C and the mitogen-activated protein kinase signaling cascade.Journal of neurochemistry · 2000Article
- Localization of a peripheral membrane protein: Gbetagamma targets Galpha(Z).Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Gi class of heterotrimeric G proteins has been implicated in transmitting mitogenic signals from a variety of seven-transmembrane domain receptors. In addition, the alpha subunit of Gi2 (alpha i2) is oncogenic when mutated to a constitutively active form (gip2). The mechanism by which Gi2 stimulates cellular proliferation is unknown, but is believed to involve activation of the mitogen-activated protein kinase (MAPK) signaling cascade. To study Gi2 activation of the cascade, we transiently expressed a mutant, pertussis toxin (PTX)-resistant alpha i2 in Chinese hamster ovary cells. After PTX treatment of these cells, Gi-coupled receptors specifically activated PTX-resistant Gi2 without activating other Gi proteins. Receptor-mediated activation of Gi2 led to activation of MAPK and its upstream activator, MAPK/ERK-activating kinase (MEK). Activation of MAPK and MEK by Gi2 was blocked by expression of a dominant-negative mutant of Ras. Gi2 activation did not, however, detectably increase the proportion of Ras protein in the GTP-bound form. Additional experiments suggest that Gi2 stimulates the MAPK pathway, at least in part, by mechanisms that involve release of its beta gamma subunit, as well as activation of phosphatidylinositol-3 kinase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.