ArticleCurrent genetics1995
Investigation of plant organellar DNAs by pulsed-field gel electrophoresis.
Article in Current genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A novel in-situ-process technique constructs whole circular cpDNA library.Plant methods · 2024Article
- Changes in DNA damage, molecular integrity, and copy number for plastid DNA and mitochondrial DNA during maize development.Journal of experimental botany · 2014Article
- Article
- The Bryopsis hypnoides plastid genome: multimeric forms and complete nucleotide sequence.PloS one · 2011Article
- RecA maintains the integrity of chloroplast DNA molecules in Arabidopsis.Journal of experimental botany · 2010Article
- Flow cytometric analysis of mitochondrial populations in HL-CMS systems of rice under H2O2 stress.Protoplasma · 2010Article
- Loss or retention of chloroplast DNA in maize seedlings is affected by both light and genotype.Planta · 2006Article
- Linear molecules of tobacco ptDNA end at known replication origins and additional loci.Plant molecular biology · 2006Article
- Changes in chloroplast DNA during development in tobacco, Medicago truncatula, pea, and maize.Planta · 2006Article
- Characterization of a mitochondrially targeted single-stranded DNA-binding protein in Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2005Article
- Circular chloroplast chromosomes: the grand illusion.The Plant cell · 2004Review
- Mitochondrial genome diversity: evolution of the molecular architecture and replication strategy.Current genetics · 2003Review
- Maize BMS cultured cell lines survive with massive plastid gene loss.Current genetics · 2003Article
- PCR analysis of pulsed-field gel electrophoresis-purified plastid DNA, a sensitive tool to judge the hetero-/homoplastomic status of plastid transformants.Current genetics · 2003Article
- The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats.The Plant cell · 2002Article
- Cytogenomic analyses reveal the structural plasticity of the chloroplast genome in higher plants.The Plant cell · 2001Article
- Organization and expression of the mitochondrial genome in the Nicotiana sylvestris CMSII mutant.Genetics · 1998Article
- High content, size and distribution of single-stranded DNA in the mitochondria of Chenopodium album (L.).Plant molecular biology · 1997Article
- Unique features of the mitochondrial rolling circle-plasmid mp1 from the higher plant Chenopodium album (L.).Nucleic acids research · 1997Article
- Rolling-circle replication of mitochondrial DNA in the higher plant Chenopodium album (L.).Molecular and cellular biology · 1996Article
Corrections and comments
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial (mt) DNAs from several higher-plant species (Arabidopsis thaliana, Beta vulgaris, Brassica hirta, Chenopodium album, Oenothera berteriana, Zea mays) were separated by pulsed-field gel electrophoresis (PFGE). Hybridization of the separated DNA with mtDNA-specific probes revealed an identical distribution of mtDNA sequences in all cases: part of the DNA formed a smear of linear molecules migrating into the gel, the rest remained in the well. Hybridization signals in the compression zone of the gels disappeared after RNase or alkaline treatment. It was shown that the linear molecules are not products of unspecific degradation by nucleases. All plastid (pt) DNA from leaves of Nicotiana tabacum remained in the well after PFGE. Separation of linear monomers and oligomers of the chloroplast chromosomes of N. tabacum was achieved by mild DNase treatment of the well-bound DNA. DNase treatment of well-bound mtDNA, however, generated a smear of linear molecules. PtDNA from cultured cells of C. album was found after PFGE to be partly well-bound, and partly separated into linear molecules with sizes of monomeric and oligomeric chromosomes. The ease with which it was possible to detect large linear molecules of plastid DNA indicates that shearing forces alone can not explain the smear of linear molecules obtained after PFGE of mtDNA. The results are discussed in relation to the structural organization of the mt genome of higher plants.
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