ArticleMolecular biology of the cell1995
Motor activity and mitotic spindle localization of the Drosophila kinesin-like protein KLP61F.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Mechanisms by Which Kinesin-5 Motors Perform Their Multiple Intracellular Functions.International journal of molecular sciences · 2021Review
- Article
- A 'molecular guillotine' reveals the interphase function of Kinesin-5.Journal of cell science · 2018Article
- Proteomic and functional analysis of the mitotic Drosophila centrosome.The EMBO journal · 2010Article
- Purification and assay of mitotic motors.Methods (San Diego, Calif.) · 2010Review
- Tyrosines in the kinesin-5 head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.Current biology : CB · 2009Article
- Kinesin-5 in Drosophila embryo mitosis: sliding filament or spindle matrix mechanism?Cell motility and the cytoskeleton · 2009Article
- MLN8054, a small-molecule inhibitor of Aurora A, causes spindle pole and chromosome congression defects leading to aneuploidy.Molecular and cellular biology · 2007Article
- Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells.Molecular biology of the cell · 2005Article
- Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster.The Journal of cell biology · 2000Article
- mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.The Journal of cell biology · 1999Article
- The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles.The Journal of cell biology · 1999Article
- Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Kinesin light chains are essential for axonal transport in Drosophila.The Journal of cell biology · 1998Article
- Characterization of the KIF3C neural kinesin-like motor from mouse.Molecular biology of the cell · 1998Article
- XCTK2: a kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts.The Journal of cell biology · 1997Article
- An essential bipolar mitotic motor.Nature · 1996Article
- A bipolar kinesin.Nature · 1996Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The KLP61F gene product is essential for Drosophila development. Mutations in KLP61F display a mitotic arrest phenotype caused by a failure in the proper separation of duplicated centrosomes (Heck et al., 1993). Sequence analysis of KLP61F identified it as a member of the bimC family of kinesin-like microtubule motor proteins. Here we report that KLP61F is distinct from KRP130, a kinesin-like protein recently purified from Drosophila embryos and suggested to be the product of the KLP61F gene (Cole et al., 1994). We also characterized recombinant KLP61F and found that it possesses microtubule-stimulated ATPase and microtubule translocation activities in vitro. In addition, we have used an affinity-purified, KLP61F-specific antiserum to localize native KLP61F and an epitope-tagged KLP61F fusion protein during various stages of mitosis in Drosophila syncytial blastoderm embryos. From early prophase through anaphase, KLP61F is coincident with the distribution of tubulin. Together these results confirm the existence of multiple bimC-like kinesins in Drosophila and suggest that KLP61F function is intrinsic to the mitotic spindle.
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