ArticlePlant physiology1996
The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Tryptophan metabolism and plant health: mechanisms of action and application prospects.Frontiers in plant science · 2026Pooled it
- Decreased Expression of a Phosphoribosylanthranilate Transferase-Encoding Gene,International journal of molecular sciences · 2025Article
- Evolution of aromatic amino acid metabolism in plants: a key driving force behind plant chemical diversity in aromatic natural products.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism.Science advances · 2024Article
- Recent advances in auxin biosynthesis and homeostasis.3 Biotech · 2023Review
- Diversification of JAZ-MYC signaling function in immune metabolism.The New phytologist · 2023Article
- Point mutations that boost aromatic amino acid production and COScience advances · 2022Article
- The entry reaction of the plant shikimate pathway is subjected to highly complex metabolite-mediated regulation.The Plant cell · 2021Article
- Genetic engineering to improve essential and conditionally essential amino acids in maize: transporter engineering as a reference.Transgenic research · 2021Article
- A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense.Plant physiology · 2020Article
- Enhanced Production of Aromatic Amino Acids in Tobacco Plants Leads to Increased Phenylpropanoid Metabolites and Tolerance to Stresses.Frontiers in plant science · 2020Article
- Evolutionary Diversification of Primary Metabolism and Its Contribution to Plant Chemical Diversity.Frontiers in plant science · 2019Review
- Fortifying Horticultural Crops with Essential Amino Acids: A Review.International journal of molecular sciences · 2017Review
- A Regulatory Hierarchy of the Arabidopsis Branched-Chain Amino Acid Metabolic Network.The Plant cell · 2017Article
- TrpE feedback mutants reveal roadblocks and conduits toward increasing secondary metabolism in Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2016Article
- Something Old, Something New: Conserved Enzymes and the Evolution of Novelty in Plant Specialized Metabolism.Plant physiology · 2015Review
- Tryptophan-independent auxin biosynthesis contributes to early embryogenesis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Biosynthetic pathway of terpenoid indole alkaloids in Catharanthus roseus.Frontiers of medicine · 2014Review
- The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway.BMC plant biology · 2013Article
- A genome scale metabolic network for rice and accompanying analysis of tryptophan, auxin and serotonin biosynthesis regulation under biotic stress.Rice (New York, N.Y.) · 2013Article
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2 authors.
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Abstract
The first step of tryptophan biosynthesis is catalyzed by anthranilate synthase (AS), which is normally subject to feedback inhibition by tryptophan. Three independent trp5 mutants defective in the Arabidopsis thaliana AS alpha subunit structural gene ASA1 were identified by selection for resistance to the herbicidal compound 6-methylanthranilate. In all three mutants these biochemical changes are caused by a single amino acid substitution from aspartate to asparagine at residue position 341. Compared with the enzyme from wild-type plants, the tryptophan concentration causing 50% inhibition of AS activity in the trp5 mutant increased nearly 3-fold, the apparent Km for chorismate decreased by approximately 50%, and the apparent Vmax increased 60%. As a consequence of altered AS kinetic properties, the trp5 mutants accumulated 3-fold higher soluble tryptophan than wild-type plants. However, even though the soluble tryptophan levels were increased in trp5 plants, the concentrations of five tryptophan biosynthetic proteins remained unchanged. These data are consistent with the hypothesis that the reaction catalyzed by A. thaliana AS is rate limiting for the tryptophan pathway and that accumulation of tryptophan biosynthetic enzymes is not repressed by a 3-fold excess of end product.
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