ArticlePlant physiology1996
A viral movement protein as a nuclear shuttle. The geminivirus BR1 movement protein contains domains essential for interaction with BL1 and nuclear localization.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 150 citations in OpenAlex.
- Identification and Genome Characterization of Begomovirus and Satellite Molecules Associated with Lettuce (Plants (Basel, Switzerland) · 2025Article
- Reassortments in single-stranded DNA multipartite viruses: Confronting expectations based on molecular constraints with field observations.Virus evolution · 2024Review
- Review
- Virus-virus interactions alter the mechanical transmissibility and host range of begomoviruses.Frontiers in plant science · 2023Article
- A plant-specific syntaxin-6 protein contributes to the intracytoplasmic route for the begomovirus CabLCV.Plant physiology · 2021Article
- Localization of Tobacco Yellow Dwarf Virus Replication Using the In Plant Activation (INPACT) Expression Platform.Viruses · 2020Article
- A Begomovirus Nuclear Shuttle Protein-Interacting Immune Hub: Hijacking Host Transport Activities and Suppressing Incompatible Functions.Frontiers in plant science · 2020Review
- A Novel Effector Protein of Apple Proliferation Phytoplasma Disrupts Cell Integrity ofInternational journal of molecular sciences · 2019Article
- A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein.Journal of virology · 2019Article
- Virus-induced gene silencing (VIGS) inPlant methods · 2019Article
- Plant Antiviral Immunity Against Geminiviruses and Viral Counter-Defense for Survival.Frontiers in microbiology · 2019Review
- Sustained NIK-mediated antiviral signalling confers broad-spectrum tolerance to begomoviruses in cultivated plants.Plant biotechnology journal · 2015Article
- Interaction between the transcription factor AtTIFY4B and begomovirus AL2 protein impacts pathogenicity.Plant molecular biology · 2014Article
- The Arabidopsis synaptotagmin SYTA regulates the cell-to-cell movement of diverse plant viruses.Frontiers in plant science · 2014Article
- Geminivirus protein structure and function.Molecular plant pathology · 2013Review
- Complete nucleotide sequence of watermelon chlorotic stunt virus originating from Oman.Viruses · 2012Article
- The induction of stromule formation by a plant DNA-virus in epidermal leaf tissues suggests a novel intra- and intercellular macromolecular trafficking route.Frontiers in plant science · 2012Article
- Evolutionary and molecular aspects of Indian tomato leaf curl virus coat protein.International journal of plant genomics · 2012Article
- Antibegomoviral activity of the agrobacterial virulence protein VirE2.Virus genes · 2011Article
- Intracellular transport of plant viruses: finding the door out of the cell.Molecular plant · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the nuclear replicating bipartite geminiviruses such as squash leaf curl to systemically infect the host requires the active participation of two virus-encoded movement proteins, BR1 and BL1. These act in a cooperative manner to transport the viral single-stranded DNA genome from its site of replication in the nucleus to the cell periphery (A.A. Sanderfoot, S.G. Lazarowitz [1995] Plant Cell 7: 1185-1194). We have proposed that BR1 functions as a nuclear shuttle protein, transporting the viral single-stranded DNA to and from the nucleus as a complex that is recognized by BL1 for movement to adjacent cells. To further investigate this, we expressed BR1 mutants known to affect viral infectivity in Spodoptera frugiperda insect cells and Nicotiana tabacum L. cv Xanthi protoplasts and found these to be defective in either their nuclear targeting or their ability to be redirected to the cell periphery when co-expressed with BL1. Translational fusions to beta-glucuronidase and alanine-scanning mutagenesis further demonstrated that the C-terminal 86 amino acids of BR1 contains a domain(s) essential for its interaction with BL1 and identified two nuclear localization signals within the N-terminal 113 residues of BR1. These nuclear localization signals were precisely located within distinct 16- and 22-peptide segments of BR1. These studies support and extend our model for squash leaf curl virus movement, showing that BR1 has a domain structure, with an N-terminal region required for nuclear targeting and a C-terminal region required for its interaction with BL1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.