ArticlePflugers Archiv : European journal of physiology1996
Cysteine-modifying reagents alter the gating of the rat cloned potassium channel Kv1.4.
Article in Pflugers Archiv : European journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Redox Regulation of KAntioxidants & redox signaling · 2024Review
- Oxidation of K(+) Channels in Aging and Neurodegeneration.Aging and disease · 2016Review
- The Human Microcirculation: Regulation of Flow and Beyond.Circulation research · 2016Review
- S-glutathionylation of an auxiliary subunit confers redox sensitivity to Kv4 channel inactivation.PloS one · 2014Article
- Cysteines control the N- and C-linker-dependent gating of KCNH1 potassium channels.Biochimica et biophysica acta · 2012Article
- Redox sensitive calcium stores underlie enhanced after hyperpolarization of aged neurons: role for ryanodine receptor mediated calcium signaling.Journal of neurophysiology · 2010Article
- Characterization of voltage-gated potassium channels in human neural progenitor cells.PloS one · 2009Article
- Oxidative regulation of large conductance calcium-activated potassium channels.The Journal of general physiology · 2001Article
- Acceleration of P/C-type inactivation in voltage-gated K(+) channels by methionine oxidation.Biophysical journal · 2000Article
- O(2) deprivation inhibits Ca(2+)-activated K(+) channels via cytosolic factors in mice neocortical neurons.The Journal of clinical investigation · 1999Article
- Transient potassium currents regulate the discharge patterns of dorsal cochlear nucleus pyramidal cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Oxidation regulates cloned neuronal voltage-dependent Ca2+ channels expressed in Xenopus oocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Modulation of potassium channel function by methionine oxidation and reduction.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Redox modulation of hslo Ca2+-activated K+ channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Effects of reducing agents and oxidants on excitation-contraction coupling in skeletal muscle fibres of rat and toad.The Journal of physiology · 1996Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of cysteine-modifying reagents on the gating of rat cloned Kv1.4 channels expressed in HEK-293 cells were examined using the whole-cell patch-clamp technique. Cells transfected with Kv1.4 expressed a rapidly inactivating K+ current with a mid-point of activation of -31 mV and a slope factor of 5 mV measured with tail current protocols in 35 mM Rb+ external solutions. The cysteine-specific oxidizing agents 2,2'-dithiobis-5-nitropyridine (DTBNP, 50 microM) and chloramine-T (CL-T, 500 microM) removed inactivation of Kv1.4. These effects were reversed by the reducing agent dithiothreitol (DTT, 10mM). In addition, DTBNP and CL-T also slowed Kv1.4 deactivation and increased the voltage sensitivity of deactivation. The action of cysteine-modifying reagents on Kv1.4 suggests that redox state affects channel gating, with oxidation tending to stabilize the open state of the channel, both by removing inactivation and slowing deactivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.