ArticlePflugers Archiv : European journal of physiology1996
A volume-activated anion conductance in insulin-secreting cells.
Article in Pflugers Archiv : European journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 97 citations in OpenAlex.
- LRRC8 channel complexes counterbalance KATP channels to mediate swell-secretion coupling in mouse pancreatic β cells.JCI insight · 2025Article
- How hypoxia-induced diabetes develops and is maintained in children born preterm.Frontiers in endocrinology · 2025Review
- Mechanosensing in Metabolism.Comprehensive Physiology · 2023Review
- Beta-Cell Ion Channels and Their Role in Regulating Insulin Secretion.Comprehensive Physiology · 2021Review
- Cell Death Induction and Protection by Activation of Ubiquitously Expressed Anion/Cation Channels. Part 1: Roles of VSOR/VRAC in Cell Volume Regulation, Release of Double-Edged Signals and Apoptotic/Necrotic Cell Death.Frontiers in cell and developmental biology · 2020Review
- Chloride transporters and channels in β-cell physiology: revisiting a 40-year-old model.Biochemical Society transactions · 2019Review
- CysLT1 receptor antagonists pranlukast and zafirlukast inhibit LRRC8-mediated volume regulated anion channels independently of the receptor.American journal of physiology. Cell physiology · 2019Article
- LRRC8/VRAC anion channels enhance β-cell glucose sensing and insulin secretion.Nature communications · 2018Article
- SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia.Nature communications · 2018Article
- The neuronal KScientific reports · 2017Article
- Ionic mechanisms in pancreatic β cell signaling.Cellular and molecular life sciences : CMLS · 2014Review
- Does NAD(P)H oxidase-derived H2O2 participate in hypotonicity-induced insulin release by activating VRAC in β-cells?Pflugers Archiv : European journal of physiology · 2012Article
- A dual action of saturated fatty acids on electrical activity in rat pancreatic β-cells. Role of volume-regulated anion channel and KATP channel currents.The Journal of physiology · 2011Article
- Article
- Studies of the mechanism of activation of the volume-regulated anion channel in rat pancreatic beta-cells.The Journal of membrane biology · 2009Article
- Permissive role of calcium on regulatory volume decrease in freshly isolated mouse cholangiocytes.Pflugers Archiv : European journal of physiology · 2007Article
- Aquaporin expression and cell volume regulation in the SV40 immortalized rat submandibular acinar cell line.Pflugers Archiv : European journal of physiology · 2007Article
- Is the glucose-induced phosphate flush in pancreatic islets attributable to gating of volume-sensitive anion channels?Endocrine · 2007Article
- Role of calcium in volume-activated chloride currents in a mouse cholangiocyte cell line.The Journal of membrane biology · 2007Review
- Stimulus-secretion coupling of hypotonicity-induced insulin release in BRIN-BD11 cells.Endocrine · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The whole-cell patch-clamp recording technique was used to measure volume-activated currents in K+-free solutions in RINm5F and HIT-T15 insulinoma cells and in dispersed rat islet cells. Cell swelling, induced by intracellular hypertonicity or extracellular hypotonicity, caused activation of an outwardly rectifying conductance which could be subsequently inactivated by hypertonic extracellular solutions. The conductance required adenosine 5'-triphosphate (ATP) in the pipette solution but was Ca2+ independent. Na+ and Cl- substitution studies suggested that the swelling-activated current is Cl- selective with a halide permeability sequence of Br > Cl > I. The conductance was reversibly inhibited by the anion channel inhibitors 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) and by 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB). Further evidence for a volume-activated anion conductance was provided by studies of volume regulation in insulin-secreting cells. When RINm5F cells were exposed to a hypotonic medium, the initial cell swelling was followed by a regulatory volume decrease (RVD). This RVD response was also inhibited by DIDS and by NPPB. These data therefore provide evidence for a volume-activated anion conductance in insulin-secreting cells which could be involved in the RVD following osmotic stress. A possible role for the conductance in hypotonically induced insulin release is also discussed.
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