ArticleGenetics1995
Requirements for activity of the yeast mitotic recombination hotspot HOT1: RNA polymerase I and multiple cis-acting sequences.
Article in Genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Genome-wide analysis of DNA replication and DNA double-strand breaks using TrAEL-seq.PLoS biology · 2021Article
- Proteins and RNA sequences required for the transition of the t-Utp complex into the SSU processome.FEMS yeast research · 2019Article
- Widespread Impact of Chromosomal Inversions on Gene Expression Uncovers Robustness via Phenotypic Buffering.Molecular biology and evolution · 2016Article
- Mechanism of regulation of 'chromosome kissing' induced by Fob1 and its physiological significance.Genes & development · 2015Article
- Physiology of the read-write genome.The Journal of physiology · 2014Review
- Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery.Nucleic acids research · 2011Article
- Can eukaryotic cells monitor the presence of unreplicated DNA?Cell division · 2007Article
- Replication fork barriers: pausing for a break or stalling for time?EMBO reports · 2007Review
- Expression of rRNA genes and nucleolus formation at ectopic chromosomal sites in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2006Article
- DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2005Article
- Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork.Genes & development · 2005Article
- SCH9, a putative protein kinase from Saccharomyces cerevisiae, affects HOT1-stimulated recombination.Molecular genetics and genomics : MGG · 2004Article
- The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork.Molecular and cellular biology · 2003Article
- Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing.Genes & development · 2003Article
- Transcription-dependent recombination and the role of fork collision in yeast rDNA.Genes & development · 2003Article
- Sir2p suppresses recombination of replication forks stalled at the replication fork barrier of ribosomal DNA in Saccharomyces cerevisiae.Nucleic acids research · 2003Article
- Transcription and double-strand breaks induce similar mitotic recombination events in Saccharomyces cerevisiae.Genetics · 2002Article
- The connection between transcription and genomic instability.The EMBO journal · 2002Review
- Yeast RNA polymerase I enhancer is dispensable for transcription of the chromosomal rRNA gene and cell growth, and its apparent transcription enhancement from ectopic promoters requires Fob1 protein.Molecular and cellular biology · 2001Article
- Identification of DNA cis elements essential for expansion of ribosomal DNA repeats in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
When inserted at novel locations in the yeast genome, the Saccharomyces cerevisiae recombination hotspot HOT1 stimulates mitotic exchange in adjacent sequences. HOT1 is derived from the rDNA repeat unit, and the sequences required for the recombination-stimulatory activity closely correspond to the rDNA transcription enhancer and initiation site, suggesting there is an association between high levels of RNA polymerase I transcription and increased recombination. To directly test whether RNA polymerase I is essential for HOT1 activity, a subunit of RNA polymerase I was deleted in a strain in which rRNA is transcribed by RNA polymerase II. HOT1 is completely inactive in this strain. Deletion analysis and site-directed mutagenesis were used to further define the sequences within the rDNA enhancer required for HOT1 activity. These studies show that the enhancer contains at least four distinct regions that are required for hotspot activity. In most cases mutations in these regions also decrease transcription from this element, further confirming the association of recombination and transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.