ArticleGenetics1995
vph6 mutants of Saccharomyces cerevisiae require calcineurin for growth and are defective in vacuolar H(+)-ATPase assembly.
Article in Genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- Oxidative Stress Responses and Nutrient Starvation in MCHM TreatedG3 (Bethesda, Md.) · 2020Article
- Vacuolar proton-translocating ATPase is required for antifungal resistance and virulence of Candida glabrata.PloS one · 2019Article
- Effects of Disruption of PMC1 in the tfp1∆/∆ Mutant on Calcium Homeostasis, Oxidative and Osmotic Stress Resistance in Candida albicans.Mycopathologia · 2018Article
- Compensatory Internalization of Pma1 in V-ATPase Mutants inGenetics · 2018Article
- Characterization of calcineurin from Cryptococcus humicola and the application of calcineurin in aluminum tolerance.BMC biotechnology · 2017Article
- Cu/Zn superoxide dismutase and the proton ATPase Pma1p of Saccharomyces cerevisiae.Biochemical and biophysical research communications · 2015Article
- Unisexual reproduction reverses Muller's ratchet.Genetics · 2014Article
- Essential role for vacuolar acidification in Candida albicans virulence.The Journal of biological chemistry · 2013Article
- Rapamycin exerts antifungal activity in vitro and in vivo against Mucor circinelloides via FKBP12-dependent inhibition of Tor.Eukaryotic cell · 2012Article
- Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics.PloS one · 2011Article
- On the Roles of Calcineurin in Fungal Growth and Pathogenesis.Current fungal infection reports · 2010Article
- Signalling pathways in the pathogenesis of Cryptococcus.Cellular microbiology · 2009Review
- A genomic screen for yeast vacuolar membrane ATPase mutants.Genetics · 2005Article
- FKBP12 controls aspartate pathway flux in Saccharomyces cerevisiae to prevent toxic intermediate accumulation.Eukaryotic cell · 2004Article
- Yeast Mn2+ transporter, Smf1p, is regulated by ubiquitin-dependent vacuolar protein sorting.Genetics · 2004Article
- Calcineurin is essential for survival during membrane stress in Candida albicans.The EMBO journal · 2002Article
- Calcium binding is required for calmodulin function in Aspergillus nidulans.Eukaryotic cell · 2002Article
- Calcineurin is required for hyphal elongation during mating and haploid fruiting in Cryptococcus neoformans.The EMBO journal · 2001Article
- Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans.The EMBO journal · 2000Article
- Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology.Molecular biology of the cell · 2000Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have characterized a Saccharomyces cerevisiae mutant strain that is hypersensitive to cyclosporin A (CsA) and FK506, immunosuppressants that inhibit calcineurin, a serine-threonine-specific phosphatase (PP2B). A single nuclear mutation, designated cev1 for calcineurin essential for viability, is responsible for the CsA-FK506-sensitive phenotype. The peptidyl-prolyl cis-trans isomerases cyclophilin A and FKBP12, respectively, mediate CsA and FK506 toxicity in the cev1 mutant strain. We demonstrate that cev1 is an allele of the VPH6 gene and that vph6 mutant strains fail to assemble the vacuolar H(+)-ATPase (V-ATPase). The VPH6 gene was mapped on chromosome VIII and is predicted to encode a 181-amino acid (21 kD) protein with no identity to other known proteins. We find that calcineurin is essential for viability in many mutant strains with defects in V-ATPase function or vacuolar acidification. In addition, we find that calcineurin modulates extracellular acidification in response to glucose, which we propose occurs via calcineurin regulation of the plasma membrane H(+)-ATPase PMA1. Taken together, our findings suggest calcineurin plays a general role in the regulation of cation transport and homeostasis.
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