ArticleBritish journal of pharmacology1995
PPADS: an antagonist at endothelial P2Y-purinoceptors but not P2U-purinoceptors.
Article in British journal of pharmacology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 71 citations in OpenAlex.
- 2',3'-O-Substituted ATP derivatives as potent antagonists of purinergic P2X3 receptors and potential analgesic agents.Purinergic signalling · 2017Article
- Nucleotide-mediated relaxation in guinea-pig aorta: selective inhibition by MRS2179.British journal of pharmacology · 2002Article
- Actions of a Series of PPADS Analogs at P2XDrug development research · 2001Article
- The pharmacology of nucleotide receptors on primary rat brain endothelial cells grown on a biological extracellular matrix: effects on intracellular calcium concentration.British journal of pharmacology · 2000Article
- Pharmacological characterization of the human P2Y11 receptor.British journal of pharmacology · 1999Article
- ATP-Induced Ca(2+) release in cochlear outer hair cells: localization of an inositol triphosphate-gated Ca(2+) store to the base of the sensory hair bundle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Synthesis and Structure-Activity Relationships of Pyridoxal-6-arylazo-5'-phosphate and Phosphonate Derivatives as P2 Receptor Antagonists.Drug development research · 1998Article
- Inhibition of ATP-activated current by zinc in dorsal root ganglion neurones of bullfrog.The Journal of physiology · 1997Article
- P2 receptor excitation of rodent hypoglossal motoneuron activity in vitro and in vivo: a molecular physiological analysis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Diadenosine polyphosphate-stimulated gluconeogenesis in isolated rat proximal tubules.The Biochemical journal · 1997Article
- Cloned and transfected P2Y4 receptors: characterization of a suramin and PPADS-insensitive response to UTP.British journal of pharmacology · 1996Article
- The P2Y purinoceptor in rat brain microvascular endothelial cells couple to inhibition of adenylate cyclase.British journal of pharmacology · 1996Article
- Co-existence of P2Y-and PPADS-insensitive P2U-purinoceptors in endothelial cells from adrenal medulla.British journal of pharmacology · 1996Article
- P2X purinoceptors in cultured myenteric neurons of guinea-pig small intestine.The Journal of physiology · 1996Article
- The effect of PPADS as an antagonist of inositol (1,4,5)trisphosphate induced intracellular calcium mobilization.British journal of pharmacology · 1996Article
- Characterization of the P2Y-purinoceptor involved in the ATP-induced rise in cytosolic Ca2+ concentration in rat ileal myocytes.British journal of pharmacology · 1996Article
- PPADS and suramin as antagonists at cloned P2Y- and P2U-purinoceptors.British journal of pharmacology · 1996Article
- Discrimination by PPADS between endothelial P2Y- and P2U-purinoceptors in the rat isolated mesenteric arterial bed.British journal of pharmacology · 1996Article
- Relative contribution of P2U- and P2Y-purinoceptors to endothelium-dependent vasodilatation in the golden hamster isolated mesenteric arterial bed.British journal of pharmacology · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Bovine aortic endothelial (BAE) cells contain two co-existing receptors for extracellular ATP, the P2Y and P2U-purinoceptors. Here we have determined whether the proposed P2X-purinoceptor antagonist, pyridoxalphosphate-6-azophenyl-2', 4'-disulphonic acid (PPADS) could distinguish between these two receptor subtypes. 2. Cells labelled with myo-[2-3H]-inositol were stimulated with increasing concentrations of either the P2Y-agonist, 2MeSATP, or the P2U-agonist, UTP in the absence or presence of 30 microM PPADS. The accumulation of total [3H]-inositol (poly)phosphates mediated by 2MeSATP was markedly attenuated by PPADS, whereas the response to UTP was not significantly affected. 3. Stimulation of BAE cells with increasing concentrations of ATP showed a reduced response in the presence of 10 microM PPADS, but this effect of the antagonist was not significant. By contrast, inhibition of the response to ADP was profound and highly significant. 4. These observations show that PPADS is not a selective P2X-purinoceptor antagonist, but is able to distinguish between P2Y- and P2YU-purinoceptors in BAE cells, and indicate that this compound may provide a useful tool in the study of multiple subtypes of P2-purinoceptors. Furthermore the results are consistent with the hypothesis that ATP interacts with both receptor subtypes, but that the action of ADP is primarily at the P2Y-purinoceptor in these endothelial cells.
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