ArticlePlanta1995
Characterisation of an mRNA encoding a metallothionein-like protein that accumulates during ethylene-promoted abscission of Sambucus nigra L. leaflets.
Article in Planta, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 82 citations in OpenAlex.
- Deep sequencing of the Mexican avocado transcriptome, an ancient angiosperm with a high content of fatty acids.BMC genomics · 2015Article
- A novel approach to dissect the abscission process in Arabidopsis.Plant physiology · 2012Article
- Programmed cell death occurs asymmetrically during abscission in tomato.The Plant cell · 2011Article
- Metallothionein-like gene from Cicer microphyllum is regulated by multiple abiotic stresses.Protoplasma · 2011Article
- Implications of metal accumulation mechanisms to phytoremediation.Environmental science and pollution research international · 2009Review
- Validation of candidate genes putatively associated with resistance to SCMV and MDMV in maize (Zea mays L.) by expression profiling.BMC plant biology · 2009Article
- The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis.Transgenic research · 2007Article
- Suppression of LX ribonuclease in tomato results in a delay of leaf senescence and abscission.Plant physiology · 2006Article
- Purification and characterization of cadmium-binding protein from unicelluar alga Chlorella sorokinian.Current microbiology · 2006Article
- Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening.Plant physiology · 2004Article
- Article
- The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm.Plant cell reports · 2004Article
- Characterization of the Arabidopsis metallothionein gene family: tissue-specific expression and induction during senescence and in response to copper.The New phytologist · 2003Article
- Differential expression of a metallothionein gene during the presymbiotic versus the symbiotic phase of an arbuscular mycorrhizal fungus.Plant physiology · 2002Article
- Temporal and spatial expression of a polygalacturonase during leaf and flower abscission in oilseed rape and Arabidopsis.Plant physiology · 2002Article
- Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins.Plant physiology · 2000Article
- Metallothioneins 1 and 2 have distinct but overlapping expression patterns in Arabidopsis.Plant physiology · 1998Article
- Structure, expression and chromosomal localisation of the metallothionein-like gene family of tomato.Plant molecular biology · 1998Article
- Differential gene expression in ripening banana fruit.Plant physiology · 1997Article
- GUS expression in Arabidopsis directed by 5' regions of the pea metallothionein-like gene PsMTA.Plant molecular biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding a metallothionein-like protein has been isolated from a cDNA library from the abscission zones of ethylene-treated Sambucus nigra leaflets. The precise function of this group of proteins in plants has yet to be confirmed but in animals there is convincing evidence that they bind heavy metals. Several of these proteins have recently been characterised from plants and it has been demonstrated that heavy metals have no stimulatory effect on their expression. In this paper we describe the isolation and characterisation of a metallothionein-like mRNA identified as a consequence of differentially screening a cDNA library for messages up-regulated during abscission. The accumulation of the mRNA occurred in the abscission zone tissue within 18 h of exposure to ethylene while, in contrast, no expression was detectable in adjacent non-abscission-zone tissue. The transcript size of the message was approximately 0.6 kb. Northern analysis revealed that the cDNA insert (JET12) did not hybridise to mRNA from either green or senescing leaflets but a signal was detectable with mRNA extracted from senescent tissue. The size of this hybridising transcript was approximately 0.5 kb. The predicted metallothionein-like protein encoded by JET12 was cysteine-rich (18.4%) and had a molecular weight of approximately 7.5 kDa. Southern analysis of S. nigra genomic DNA showed that the mRNA was encoded by a small gene family. The protein exhibited greatest homology to other metallothioneins belonging to the Type 2 family including those from Mimulus (62%) and Arabidopsis (57%). This homology was greatest around the cysteine-rich amino and carboxy termini.(ABSTRACT TRUNCATED AT 250 WORDS)
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