ArticleBiophysical journal1995
Interpreting photoactivated fluorescence microscopy measurements of steady-state actin dynamics.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.Journal of muscle research and cell motility · 2020Review
- Myosin-dependent actin stabilization as revealed by single-molecule imaging of actin turnover.Molecular biology of the cell · 2018Article
- Measurement of Rapid Protein Diffusion in the Cytoplasm by Photo-Converted Intensity Profile Expansion.Cell reports · 2017Article
- Simultaneous quantification of actin monomer and filament dynamics with modeling-assisted analysis of photoactivation.Journal of cell science · 2016Article
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- Image based validation of dynamical models for cell reorientation.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1.The Journal of cell biology · 2015Article
- Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching.Nature protocols · 2015Article
- A phenomenological density-scaling approach to lamellipodial actin dynamics(†).Interface focus · 2014Article
- Responses to cell loss become restricted as the supporting cells in mammalian vestibular organs grow thick junctional actin bands that develop high stability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Analysis of turnover dynamics of the submembranous actin cortex.Molecular biology of the cell · 2013Article
- Distributed actin turnover in the lamellipodium and FRAP kinetics.Biophysical journal · 2013Article
- A mathematical model of actin filament turnover for fitting FRAP data.European biophysics journal : EBJ · 2010Article
- Inside view of cell locomotion through single-molecule: fast F-/G-actin cycle and G-actin regulation of polymer restoration.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2010Review
- An order of magnitude faster AIP1-associated actin disruption than nucleation by the Arp2/3 complex in lamellipodia.PloS one · 2009Article
- A finite element model for protein transport in vivo.Biomedical engineering online · 2007Article
- In vivo assay of presynaptic microtubule cytoskeleton dynamics in Drosophila.Journal of neuroscience methods · 2007Article
- Identification of biomolecule mass transport and binding rate parameters in living cells by inverse modeling.Theoretical biology & medical modelling · 2006Article
- The DNA binding activity of p53 displays reaction-diffusion kinetics.Biophysical journal · 2006Article
- Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins.Biophysical journal · 2006Article
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4 authors.
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Abstract
A continuum model describing the steady-state actin dynamics of the cytoskeleton of living cells has been developed to aid in the interpretation of photoactivated fluorescence experiments. In a simplified cell geometry, the model assumes uniform concentrations of cytosolic and cytoskeletal actin throughout the cell and no net growth of either pool. The spatiotemporal evolution of the fluorescent actin population is described by a system of two coupled linear partial-differential equations. An analytical solution is found using a Fourier-Laplace transform and important limiting cases relevant to the design of experiments are discussed. The results demonstrate that, despite being a complex function of the parameters, the fluorescence decay in photoactivated fluorescence experiments has a biphasic behavior featuring a short-term decay controlled by monomer diffusion and a long-term decay governed by the monomer exchange rate between the polymerized and unpolymerized actin pools. This biphasic behavior suggests a convenient mechanism for extracting the parameters governing the fluorescence decay from data records. These parameters include the actin monomer diffusion coefficient, filament turnover rate, and ratio of polymerized to unpolymerized actin.
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