ArticleThe American journal of pathology1996
Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes.
Article in The American journal of pathology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 101 citations in OpenAlex.
- "Small Hepatocytes" in the Liver.Cells · 2023Review
- A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functionsRegenerative therapy · 2016Article
- Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation.Journal of visualized experiments : JoVE · 2015Article
- Micropatterned co-culture of hepatocyte spheroids layered on non-parenchymal cells to understand heterotypic cellular interactions.Science and technology of advanced materials · 2013Article
- Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.Archives of toxicology · 2013Review
- Direct differentiation of hepatic stem-like WB cells into insulin-producing cells using small molecules.Scientific reports · 2013Article
- Gene transfection to spheroid culture system on micropatterned culture plate by polyplex nanomicelle: a novel platform of genetically-modified cell transplantation.Drug delivery and translational research · 2012Article
- The MAPK MEK1/2-ERK1/2 Pathway and Its Implication in Hepatocyte Cell Cycle Control.International journal of hepatology · 2012Article
- Mesenchymal stromal cells as supportive cells for hepatocytes.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Hepatic stem cells: from inside and outside the liver?Cell proliferation · 2004Review
- Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes.Gene therapy · 2003Article
- Expression of E-cadherin and other paracellular junction genes is decreased in iron-loaded hepatocytes.The American journal of pathology · 2003Article
- Pleiotrophin/heparin-binding growth-associated molecule as a mitogen of rat hepatocytes and its role in regeneration and development of liver.The American journal of pathology · 2002Article
- Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes.Journal of virology · 2001Article
- Transduction of primary rat hepatocytes with bicistronic retroviral vector.World journal of gastroenterology · 2000Article
- Analysis of the ammonia metabolism of rat primary hepatocytes and a human hepatocyte cell line Huh 7.Cytotechnology · 2000Article
- Wound healing in the liver with particular reference to stem cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1998Review
- Growth and differentiation in culture of clonogenic hepatocytes that express both phenotypes of hepatocytes and biliary epithelial cells.The American journal of pathology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study succeeded in cultivating normal adult rat hepatocytes for at least 85 days without losing their replicative potential and differentiation capacity. Small pieces of hepatocyte aggregates (clusters) were prepared from the primary culture of hepatocytes and used as starting material for the growth experiment. Some of the hepatocytes started to proliferate at 3 days when the clusters were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 10 ng/ml epidermal growth factor, 10 mmol/L nicotinamide, 0.2 mmol/L L-ascorbic acid 2-phosphate, and 1% dimethylsulfoxide. Clusters continued to grow and formed colonies. All the cells covering colonies expressed normal hepatocyte-specific proteins. The number of albumin-expressing cells in the most replicative colonies increased sixfold during 32 days. Most of the cells were mononucleate and small in size and some of them expressed immature hepatocyte markers such as alpha-fetoprotein. Electron microscopy of cells in colonies revealed the presence of peroxisomes in the cytoplasm and desmosomes, tight junctions, and bile canaliculus-like structures between the cells. Depletion of one of the additives inhibited the growth of hepatocytes. The culture medium used also supported the growth of stellate cells (Ito cells) that had contaminated the original preparation in small numbers and seems to cooperatively stimulate a proliferative population of hepatocytes.
Indexed as
Identifiers
8579101PMC1861672W186151347What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.