ArticleProceedings of the National Academy of Sciences of the United States of America1996
Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 203 papers.
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Who cites it
203 citing papers in PubMed, 801 citations in OpenAlex.
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- Construction and Stability of All-in-One Adenovirus Vectors Simultaneously Expressing Four and Eight Multiplex Guide RNAs and Cas9 Nickase.International journal of molecular sciences · 2024Article
- DYRK2 promotes chemosensitivity via p53-mediated apoptosis after DNA damage in colorectal cancer.Cancer science · 2023Article
- Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector.Scientific reports · 2023Article
- A recombinant adenovirus vector containing theFrontiers in oncology · 2023Article
- Enforced dual-specificity tyrosine-regulated kinase 2 expression by adenovirus-mediated gene transfer inhibits tumor growth and metastasis of colorectal cancer.Cancer science · 2022Article
- Review
- The physiological role of Homer2a and its novel short isoform, Homer2e, in NMDA receptor-mediated apoptosis in cerebellar granule cells.Molecular brain · 2021Article
- Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing.Scientific reports · 2021Article
- Overexpression of SOCS3 mediated by adenovirus vector in mouse and human castration-resistant prostate cancer cells increases the sensitivity to NK cells in vitro and in vivo.Cancer gene therapy · 2019Article
- ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling.Cell communication and signaling : CCS · 2019Article
- Silencing of both ATF4 and PERK inhibits cell cycle progression and promotes the apoptosis of differentiating chondrocytes.International journal of molecular medicine · 2017Article
- Pattern of RECK CpG methylation as a potential marker for predicting breast cancer prognosis and drug-sensitivity.Oncotarget · 2016Article
- Article
- Preferable sites and orientations of transgene inserted in the adenovirus vector genome: The E3 site may be unfavorable for transgene position.Gene therapy · 2015Article
- A phase 1 study of a heterologous prime-boost vaccination involving a truncated HER2 sequence in patients with HER2-expressing breast cancer.Molecular therapy. Methods & clinical development · 2015Article
- Deficiency of GDNF Receptor GFRα1 in Alzheimer's Neurons Results in Neuronal Death.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Development of a novel efficient method to construct an adenovirus library displaying random peptides on the fiber knob.Molecular pharmaceutics · 2014Article
- Differential regulation of mouse pancreatic islet insulin secretion and Smad proteins by activin ligands.Diabetologia · 2014Article
- Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery.Scientific reports · 2013Article
143 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the full-length Ad genome cloned in a cassette cosmid. The cassette bearing the expression unit was then cotransfected into human embryonic kidney 293 cells together with the Ad DNA-terminal protein complex digested at several sites with Eco T22I or Ase I/EcoRI. The use of the parent Ad DNA-terminal protein complex instead of the deproteinized Ad genome DNA allowed very efficient recovery of the desired recombinant Ad, and the above restriction digestion drastically reduced regeneration of the parent virus. Several hundred virus clones were readily obtained in each experiment, and about 70% of the clones were the desired recombinant viruses. Furthermore, because the cassette contained the full-length Ad genome, any position of the genome could be easily modified to develop a new vector design. We established construction systems for two types of Ad vectors, the E1-substitution type and the E4-insertion type. This method may greatly facilitate the application of recombinant Ads and should be useful for further improvement of Ad vectors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.