ArticleProceedings of the National Academy of Sciences of the United States of America1996
Lens complementation system for the genetic analysis of growth, differentiation, and apoptosis in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Blastocyst complementation: current progress and future directions in xenogeneic organogenesis.Stem cell research & therapy · 2025Review
- Chimeric Livers: Interspecies Blastocyst Complementation and Xenotransplantation for End-Stage Liver Disease.Hepatic medicine : evidence and research · 2024Review
- In vitro and in vivo functions of T cells produced in complemented thymi of chimeric mice generated by blastocyst complementation.Scientific reports · 2022Article
- Article
- The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras.Development (Cambridge, England) · 2021Article
- Direct analysis of brain phenotypes via neural blastocyst complementation.Nature protocols · 2020Article
- Pluripotent stem cell-derived organogenesis in the rat model system.Transgenic research · 2019Review
- Absent forebrain replaced by embryonic stem cells.Nature · 2018Article
- Article
- Interspecies chimeric complementation for the generation of functional human tissues and organs in large animal hosts.Transgenic research · 2016Review
- Mouse chimeras as a system to investigate development, cell and tissue function, disease mechanisms and organ regeneration.Cell cycle (Georgetown, Tex.) · 2011Review
- An essential role for FGF receptor signaling in lens development.Seminars in cell & developmental biology · 2006Review
- Cell cycle regulation in the developing lens.Seminars in cell & developmental biology · 2006Review
- Fibroblast growth factor receptor 1 (Fgfr1) is not essential for lens fiber differentiation in mice.Molecular vision · 2006Article
- Cooperation between the Cdk inhibitors p27(KIP1) and p57(KIP2) in the control of tissue growth and development.Genes & development · 1998Article
- A Technological and Regulatory Review on Human-Animal Chimera Research: The Current Landscape of Biology, Law, and Public Opinion.Cell transplantationReview
- Interspecies Organogenesis for Human Transplantation.Cell transplantationReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A genetic approach has been established that combines the advantages of blastocyst complementation with the experimental attributes of the developing lens for the functional analysis of genes governing cellular proliferation, terminal differentiation, and apoptosis. This lens complementation system (LCS) makes use of a mutant mouse strain, aphakia (ak), homozygotes of which fail to develop an ocular lens. We demonstrate that microinjection of wild-type embryonic stem (ES) cells into ak/ak blastocysts produces chimeras with normal ES-cell-derived lenses and that microinjection of Rb-/- ES cells generates an aberrant lens phenotype identical to that obtained through conventional gene targeting methodology. Our determination that a cell autonomous defect underlies the aphakia condition assures that lenses generated through LCS are necessarily ES-cell-derived. LCS provides for the rapid phenotypic analysis of loss-of-function mutations, circumvents the need for germ-line transmission of null alleles, and, most significantly, facilitates the study of essential genes whose inactivation is associated with early lethal phenotypes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.