ArticleMolecular biology of the cell1995
The actin-related protein Act3p of Saccharomyces cerevisiae is located in the nucleus.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 63 citations in OpenAlex.
- Actin, actin-binding proteins, and actin-related proteins in the nucleus.Histochemistry and cell biology · 2016Review
- Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla.Frontiers in plant science · 2016Article
- Actin-related protein Arp6 influences H2A.Z-dependent and -independent gene expression and links ribosomal protein genes to nuclear pores.PLoS genetics · 2010Article
- Cooperation between the INO80 complex and histone chaperones determines adaptation of stress gene transcription in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2009Article
- Chapter 5. Nuclear actin-related proteins in epigenetic control.International review of cell and molecular biology · 2009Review
- ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis.Plant & cell physiology · 2008Article
- Actin-related protein Arp4 functions in kinetochore assembly.Nucleic acids research · 2007Article
- Sequence and comparative genomic analysis of actin-related proteins.Molecular biology of the cell · 2005Article
- Fission yeast Arp6 is required for telomere silencing, but functions independently of Swi6.Nucleic acids research · 2004Article
- Remodeling of organelle-bound actin is required for yeast vacuole fusion.The Journal of cell biology · 2002Article
- Correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae.Nucleic acids research · 2002Article
- Further characterization of the actin-related protein Act3p/Arp4 of S. cerevisiae through mutational analysis.Molecular biology reports · 2000Article
- The Arabidopsis actin-related protein 2 (AtARP2) promoter directs expression in xylem precursor cells and pollen.Plant molecular biology · 1999Article
- The nuclear actin-related protein of Saccharomyces cerevisiae, Act3p/Arp4, interacts with core histones.Molecular biology of the cell · 1999Article
- Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles.The Journal of cell biology · 1996Article
- Actin-related proteins localized in the nucleus: from discovery to novel roles in nuclear organization.Nucleus (Austin, Tex.)Review
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Actin-related proteins, a group of protein families that exhibit about 50% sequence identity among each other and to conventional actin, have been found in a variety of eukaryotic organisms. In the budding yeast Saccharomyces cerevisiae, genes for one conventional actin (ACT1) and for three actin-related proteins (ACT2, ACT3, and ACT5) are known. ACT3, which we recently discovered, is an essential gene coding for a polypeptide of 489 amino acids (Act3p), with a calculated molecular mass of 54.8 kDa. Besides its homology to conventional actin, Act3p possesses a domain exhibiting weak similarity to the chromosomal protein HMG-14 as well as a potential nuclear localization signal. An antiserum prepared against a specific segment of the ACT3 gene product recognizes a polypeptide band of approximately 55 kDa in yeast extract. Indirect immunofluorescence experiments with this antiserum revealed that Act3p is located in the nucleus. Nuclear staining was observed in all cells regardless of the stage of the cell cycle. Independently, immunoblotting experiments with subcellular fractions showed that Act3p is indeed highly enriched in the nuclear fraction. We suggest that Act3p is an essential constituent of yeast chromatin.
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