ArticleThe Biochemical journal1996
Phospholipase D activation by P2Z-purinoceptor agonists in human lymphocytes is dependent on bivalent cation influx.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 70 citations in OpenAlex.
- P2X7 Receptor in Dendritic Cells and Macrophages: Implications in Antigen Presentation and T Lymphocyte Activation.International journal of molecular sciences · 2024Review
- P2X7-mediated alteration of membrane fluidity is associated with the late stages of age-related macular degeneration.Purinergic signalling · 2022Article
- Physiologic roles of P2 receptors in leukocytes.Journal of leukocyte biology · 2022Review
- Structural and Functional Basis for Understanding the Biological Significance of P2X7 Receptor.International journal of molecular sciences · 2020Review
- P2X7 Interactions and Signaling - Making Head or Tail of It.Frontiers in molecular neuroscience · 2019Review
- Article
- CAY10593 inhibits the human P2X7 receptor independently of phospholipase D1 stimulation.Purinergic signalling · 2013Article
- Lipid metabolism modulation by the P2X7 receptor in the immune system and during the course of infection: new insights into the old view.Purinergic signalling · 2011Article
- Large-conductance channel formation mediated by P2X7 receptor activation is regulated through distinct intracellular signaling pathways in peritoneal macrophages and 2BH4 cells.Naunyn-Schmiedeberg's archives of pharmacology · 2010Article
- P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Wounding sheets of epithelial cells activates the epidermal growth factor receptor through distinct short- and long-range mechanisms.Molecular biology of the cell · 2008Article
- P2X(7) receptor: Death or life?Purinergic signalling · 2005Article
- Chelerythrine and other benzophenanthridine alkaloids block the human P2X7 receptor.British journal of pharmacology · 2004Article
- Coupling of a P2Z-like purinoceptor to a fatty acid-activated K(+) channel in toad gastric smooth muscle cells.The Journal of physiology · 2001Article
- Activation of phospholipase D by metabotropic glutamate receptor agonists in rat cerebrocortical synaptosomes.British journal of pharmacology · 2000Article
- ATP-induced [Ca(2+)](i) changes and depolarization in GH3 cells.British journal of pharmacology · 2000Article
- Endometrial cell death during early pregnancy in the rat.The Histochemical journal · 2000Article
- P2 receptor subtypes in the cardiovascular system.The Biochemical journal · 1998Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of bivalent cations in ATP-stimulated phospholipase D (PLD) activity was investigated in human leukaemic lymphocytes. Cells were labelled with [3H]oleic acid and incubated with extracellular ATP or benzoylbenzoic ATP in the presence of 1 mM Ca2+ and butanol, and PLD activity was assayed by the accumulation of [3H]phosphatidylbutanol ([3H]PBut). ATP stimulated PLD activity in a dose-dependent manner, and the inhibitory effects of suramin, oxidized ATP and extracellular Mg2+ suggested that the effect of ATP was mediated by P2Z purinoceptors known to be present on lymphocytes. Thapsigargin increased cytosolic [Ca2+] but did not stimulate PLD activity, whereas preloading cells with a Ca2+ chelator reduced cytosolic [Ca2+] and, paradoxically, potentiated ATP-stimulated [3H]PBut accumulation. ATP-stimulated [3H]PBut formation was supported by both Ba2+ and Sr2+ when they were substituted for extracellular Ca2+. Addition of EGTA to block bivalent cation influx inhibited the majority of ATP-stimulated PLD activity. Furthermore ATP-stimulated PLD activity showed a linear relationship to extracellular [Ba2+], and ATP-induced 133Ba2+ influx also had a linear dependence on extracellular [Ba2+]. These results suggest that ATP stimulates PLD activity in direct proportion to the influx of bivalent cations through the P2Z-purinoceptor ion channel and that this PLD activity is insensitive to changes in bulk cytosolic [Ca2+].
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.