ArticleProceedings of the National Academy of Sciences of the United States of America1996
Analysis of differential gene expression by display of 3' end restriction fragments of cDNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Whole transcriptome analysis with sequencing: methods, challenges and potential solutions.Cellular and molecular life sciences : CMLS · 2015Review
- Large-scale tag/PCR-based gene expression profiling.World journal of microbiology & biotechnology · 2014Review
- EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics.BMC genomics · 2014Article
- Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs.BMC genomics · 2008Article
- A genomic analysis of RNA polymerase II modification and chromatin architecture related to 3' end RNA polyadenylation.Genome research · 2008Article
- Modified serial analysis of gene expression method for construction of gene expression profiles of microbial eukaryotic species.Applied and environmental microbiology · 2004Article
- Marrow stem cells shift gene expression and engraftment phenotype with cell cycle transit.The Journal of experimental medicine · 2003Article
- An integrated computational and laboratory approach for selective amplification of mRNAs containing the adenylate uridylate-rich element consensus sequence.Genome research · 2002Article
- Review
- Digital analysis of cDNA abundance; expression profiling by means of restriction fragment fingerprinting.BMC genomics · 2002Article
- New molecular methods for classification, diagnosis and therapy prediction of hematological malignancies.Pathology oncology research : POR · 2002Review
- Comprehensive gene expression analysis by transcript profiling.Plant molecular biology · 2002Review
- A critical evaluation of differential display as a tool to identify genes involved in legume nodulation: looking back and looking forward.Nucleic acids research · 2001Article
- Multiplex polymerase chain reaction (PCR) with color-tagged module-shuffling primers for comparing gene expression levels in various cells.Nucleic acids research · 2001Article
- Article
- Diversity arrays: a solid state technology for sequence information independent genotyping.Nucleic acids research · 2001Article
- Sequence analysis of TnphoA insertion sites in Vibrio cholerae mutants defective in rugose polysaccharide production.Infection and immunity · 2000Article
- Applications of differential-display reverse transcription-PCR to molecular pathogenesis and medical mycology.Clinical microbiology reviews · 2000Review
- TOGA: an automated parsing technology for analyzing expression of nearly all genes.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The coupling of differential display and AFLP approaches for nonradioactive mRNA fingerprinting.Molecular biotechnology · 1999Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an approach to study changes in gene expression by selective PCR amplification and display of 3' end restriction fragments of double-stranded cDNAs. This method produces highly consistent and reproducible patterns, can detect almost all mRNAs in a sample, and can resolve hidden differences such as bands that differ in their sequence but comigrate on a gel. Bands corresponding to known cDNAs move to predictable positions on the gel, making this a powerful approach to correlate gel patterns with cDNA data bases. Applying this method, we have examined differences in gene expression patterns during T-cell activation. Of a total of 700 bands that were evaluated in this study, as many as 3-4% represented mRNAs that are upregulated, while approximately 2% were down-regulated within 4 hr of activation of Jurkat T cells. These and other results suggest that this approach is suitable for the systematic, expeditious, and nearly exhaustive elucidation of subtle changes in the patterns of gene expression in cells with altered physiologic states.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.