ArticleMolecular & general genetics : MGG1996
Caffeine-resistance in fission yeast is caused by mutations in a single essential gene, crm1+.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Heterochromatin epimutations impose mitochondrial dysfunction to confer antifungal resistance.The EMBO journal · 2026Article
- POH1/Rpn11/PSMD14: a journey from basic research in fission yeast to a prognostic marker and a druggable target in cancer cells.British journal of cancer · 2022Review
- Yap1-mediated Flr1 expression reveals crosstalk between oxidative stress signaling and caffeine resistance inFrontiers in microbiology · 2022Article
- Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms.Journal of microbiology and biotechnology · 2021Review
- The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.The Journal of biological chemistry · 2013Article
- The CRM1 nuclear export protein in normal development and disease.International journal of biochemistry and molecular biology · 2012Article
- Article
- Oxidative stress in Schizosaccharomyces pombe: different H2O2 levels, different response pathways.Molecular genetics and genomics : MGG · 2006Review
- Cip1 and Cip2 are novel RNA-recognition-motif proteins that counteract Csx1 function during oxidative stress.Molecular biology of the cell · 2006Article
- Activation of AP-1-dependent transcription by a truncated translation initiation factor.Eukaryotic cell · 2005Article
- The transcription factor Pap1/Caf3 plays a central role in the determination of caffeine resistance in Schizosaccharomyces pombe.Molecular genetics and genomics : MGG · 2004Article
- Distinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe.Molecular biology of the cell · 2002Article
- Fission yeast Int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation.Molecular biology of the cell · 2000Article
- The Drosophila embargoed gene is required for larval progression and encodes the functional homolog of schizosaccharomyces Crm1.Genetics · 2000Article
- Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.Molecular biology of the cell · 1999Article
- Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor.The EMBO journal · 1998Article
- Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1.Genes & development · 1998Article
- The ubiquitin-proteasome pathway in cancer.British journal of cancer · 1998Review
- Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caffeine is a base analogue and is known to affect a wide variety of cellular processes. In order to dissect genetically molecules which mediate the biological effects of caffeine, temperature-sensitive (ts) and caffeine-resistant mutants were isolated from fission yeast, Schizosaccharomyces pombe. Surprisingly, all twelve ts isolates contained a mutation in the same locus, crm1. Cells of the ts crm1 mutant showed an abnormal chromosome structure at the restrictive temperature, an elevated expression of Pap1-dependent transcription, and cross-resistance to an unrelated drug such as staurosporine. Overproduction of pap1+ also conferred caffeine resistance, whilst the resistance of the crm1 mutant is abolished in the pap1- background. These results show that the crm1+ gene is a major locus for caffeine resistance, which arises from Pap1-dependent transcriptional activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.