ArticleThe Journal of membrane biology1995
A voltage-dependent and pH-sensitive proton current in Rana esculenta oocytes.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Assessing Structural Determinants of ZnBiophysical journal · 2020Article
- Voltage-gated proton channels exist in the plasma membrane of human oocytes.Human reproduction (Oxford, England) · 2019Article
- Extracellular phosphates enhance activities of voltage-gated proton channels and production of reactive oxygen species in murine osteoclast-like cells.Pflugers Archiv : European journal of physiology · 2017Article
- Acid-inducible proton influx currents in the plasma membrane of murine osteoclast-like cells.Pflugers Archiv : European journal of physiology · 2016Article
- The Voltage-Gated Proton Channel: A Riddle, Wrapped in a Mystery, inside an Enigma.Biochemistry · 2015Review
- Philosophy of voltage-gated proton channels.Journal of the Royal Society, Interface · 2014Review
- Analysis of electrophysiological properties and responses of neutrophils.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Voltage-gated proton channels: molecular biology, physiology, and pathophysiology of the H(V) family.Physiological reviews · 2013Review
- Biophysical properties of the voltage gated proton channel H(V)1.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Voltage-gated proton channels.Comprehensive Physiology · 2012Review
- Voltage-gated proton channels: what's next?The Journal of physiology · 2008Review
- Detailed comparison of expressed and native voltage-gated proton channel currents.The Journal of physiology · 2008Article
- The pH dependence of NADPH oxidase in human eosinophils.The Journal of physiology · 2005Article
- pH-dependent inhibition of voltage-gated H(+) currents in rat alveolar epithelial cells by Zn(2+) and other divalent cations.The Journal of general physiology · 1999Article
- Effects of buffer concentration on voltage-gated H+ currents: does diffusion limit the conductance?Biophysical journal · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage clamp technique was used to study macroscopic ionic currents in Rana esculenta oocytes. Depolarization steps led to the activation of a single type of outward current (Iout) when contaminant potassium and calcium-dependent chloride currents were pharmacologically inhibited. The voltage threshold of Iout activation was 10 mV and this current, which did not inactivate, presented a deactivation the time constant of 73 +/- 21 msec (n = 26) corresponding to a membrane voltage of -60 mV. Its reversal potential (Erev) was dependent on the magnitude of the depolarization and also on pulse duration. These changes in Erev were thought to reflect intracellular ion depletion occurring during activation of the remaining outward current. Furthermore, the activation threshold of Iout was clearly affected by modifications in extracellular and intracellular H+ concentrations. Indeed, intracellular alkalinization (evoked by external application of ammonium chloride) or extracellular acidification induced a rightward shift in the activation threshold while intracellular acidification (evoked by external application of sodium acetate) or extracellular alkalinization shifted this threshold toward a more negative value. Lastly, Iout was dramatically reduced by divalent cations such as Cd2+, Ni2+ or Zn2+ and was strongly decreased by 4 Aminopyridine (4-AP), well-known H+ current antagonists already described in many cell types. Therefore, it was suggested that the outward current was prominently carried by H+ ions, which may play a key role in the regulation of intracellular pH and subsequent pH dependent processes in Rana oocyte.
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