ArticleThe Journal of membrane biology1995
Volume-activated chloride currents in pancreatic duct cells.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Structure, Dynamics, and Substrate Specificity of the OprO Porin from Pseudomonas aeruginosa.Biophysical journal · 2015Article
- Regulation of bradykinin-induced activation of volume-sensitive outwardly rectifying anion channels by Ca2+ nanodomains in mouse astrocytes.The Journal of physiology · 2011Article
- Functional and molecular characterization of a volume-activated chloride channel in rabbit corneal epithelial cells.The Journal of membrane biology · 2004Article
- Human cervical cancer cells use Ca2+ signalling, protein tyrosine phosphorylation and MAP kinase in regulatory volume decrease.The Journal of physiology · 2001Article
- Signaling events during swelling and regulatory volume decrease.Neurochemical research · 2000Review
- Molecular determinants of anion selectivity in the cystic fibrosis transmembrane conductance regulator chloride channel pore.Biophysical journal · 2000Article
- Cell volume regulation: the role of taurine loss in maintaining membrane potential and cell pH.The Journal of physiology · 2000Article
- Synergetic activation of outwardly rectifying Cl- currents by hypotonic stress and external Ca2+ in murine osteoclasts.The Journal of physiology · 1999Article
- Characterization of cell volume-sensitive chloride currents in freshly prepared and cultured pancreatic acinar cells from early postnatal rats.The Journal of physiology · 1998Article
- Involvement of PKC-alpha in regulatory volume decrease responses and activation of volume-sensitive chloride channels in human cervical cancer HT-3 cells.The Journal of physiology · 1998Article
- Non-pore lining amino acid side chains influence anion selectivity of the human CFTR Cl- channel expressed in mammalian cell lines.The Journal of physiology · 1998Article
- Involvement of stretch-activated Cl- channels in ramification of murine microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Mitogen-activated protein and tyrosine kinases in the activation of astrocyte volume-activated chloride current.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Autocrine signaling through ATP release represents a novel mechanism for cell volume regulation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The role of swelling-induced anion channels during neuronal volume regulation.Molecular neurobiology · 1996Review
- Potent block of volume-activated chloride currents in endothelial cells by the uncharged form of quinine and quinidine.British journal of pharmacology · 1996Article
- Volume-sensitive chloride currents in primary cultures of human fetal vas deferens epithelial cells.Pflugers Archiv : European journal of physiology · 1996Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have used the patch clamp technique to study volume-activated Cl- currents in the bicarbonate-secreting pancreatic duct cell. These currents could be elicited by a hypertonic pipette solution (osmotic gradient 20 mOsm/l), developed over about 8 min to a peak value of 91 +/- 5.8 pA/pF at 60 mV (n = 123), and were inhibited by a hypertonic bath solution. The proportion of cells which developed currents increased from 15% in freshly isolated ducts to 93% if the ducts were cultured for 2 days. The currents were ATP-dependent, had an outwardly rectifying current/voltage (I-V) plot, and displayed time-dependent inactivation at depolarizing potentials. The anion selectivity sequence was: ClO4 = I = SCN > Br = NO3 > Cl > F > HCO3 > gluconate, and the currents were inhibited to a variable extent by DIDS, NPPB, dideoxyforskolin, tamoxifen, verapamil and quinine. Increasing the intracellular Ca2+ buffering capacity, or lowering the extracellular Ca2+ concentration, reduced the proportion of duct cells which developed currents. However, removal of extracellular Ca2+ once the currents had developed was without effect. Inhibiting protein kinase C (PKC) with either the pseudosubstrate PKC (19-36), calphostin C or staurosporine completely blocked development of the currents. We speculate that cell swelling causes Ca2+ influx which activates PKC which in turn either phosphorylates the Cl- channel or a regulatory protein leading to channel activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.