Trial reportJournal of clinical microbiology1995
Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis.
Trial report in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis.BMC infectious diseases · 2004Pooled it
- Rapid identification of Mycobacterium tuberculosis complex on urine samples by Gen-Probe amplification test.Urological research · 1997Trial
- Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients.Memorias do Instituto Oswaldo Cruz · 2014Article
- Tuberculosis of the breast.Annals of thoracic medicine · 2008Article
- Mycobacterium tuberculosis and rifampin resistance, United Kingdom.Emerging infectious diseases · 2006Article
- Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis.Journal of clinical microbiology · 2004Article
- Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples.Journal of clinical microbiology · 2003Article
- Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction.Journal of clinical microbiology · 2003Article
- Multicenter evaluation of the Abbott LCx Mycobacterium tuberculosis ligase chain reaction assay.Journal of clinical microbiology · 1999Article
- Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.Journal of clinical microbiology · 1999Article
- Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in human samples.Journal of clinical microbiology · 1999Article
- Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and nonrespiratory specimens.Journal of clinical microbiology · 1998Article
- Performance of transcription-mediated amplification and ligase chain reaction assays for detection of chlamydial infection in urogenital samples obtained by invasive and noninvasive methods.Journal of clinical microbiology · 1998Article
- Rapid diagnosis of extrapulmonary tuberculosis by ligase chain reaction amplification.Journal of clinical microbiology · 1998Article
- Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by ligase chain reaction.Journal of clinical microbiology · 1998Article
- Identification of a new DNA region specific for members of Mycobacterium tuberculosis complex.Journal of clinical microbiology · 1998Article
- Comparison of PCR, culture, and histopathology for diagnosis of tuberculous pericarditis.Journal of clinical microbiology · 1997Article
- Diagnostic value of the strand displacement amplification method compared to those of Roche Amplicor PCR and culture for detecting mycobacteria in sputum samples.Journal of clinical microbiology · 1997Article
- Evaluation of a commercial ligase chain reaction kit (Abbott LCx) for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary specimens.Journal of clinical microbiology · 1997Article
- Mycobacterium growth indicator tube testing in conjunction with the AccuProbe or the AMPLICOR-PCR assay for detecting and identifying mycobacteria from sputum samples.Journal of clinical microbiology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five hundred fifty respiratory and nonrespiratory specimens from 340 patients were analyzed by comparing the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (MTD) with conventional culture, which was the method of reference, for the detection of the Mycobacterium tuberculosis complex. After resolution of discrepant results by retesting the samples and reviewing the patients' clinical histories, a total of 60 respiratory specimens were MTD and culture positive, 347 were MTD and culture negative, 4 were MTD positive and culture negative, and 1 was MTD negative and culture positive. This results in a sensitivity of 98.4%, a specificity of 98.9%, and positive and negative predictive values of 93.8 and 99.7%, respectively. Repeatedly, clinicians asked to test specimens of nonpulmonary origin by MTD. Although, MTD is not approved for use with nonrespiratory specimens, the following results were shown. Sixty-one pleural exudate specimens showed disappointing results (sensitivity, 20%). However, MTD performed well with another 77 nonrespiratory specimens; 17 samples were positive and 57 samples were negative by both MTD and culture. No false-negative results were found by MTD. Three MTD-positive, culture-negative specimens had high sample relative light unit/cutoff relative light unit ratios, strongly suggesting true tuberculosis. Positive microscopy and positive culture with MTD-negative results occurred 12 times. Those cultures showed atypical mycobacteria 11 times and Actinomyces species once. The stability of the reagents in the MTD kit was also assessed by testing reagents, including the enzyme mixture, kept at -70 degrees C for at least 6 months. No loss of activity was seen.
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