Trial reportJournal of clinical microbiology1995
Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR.
Trial report in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 103 citations in OpenAlex.
- Pooled it
- Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis.Journal of clinical microbiology · 2003Pooled it
- Proficiency testing of clinical microbiology laboratories using modified decontamination procedures for detection of nontuberculous mycobacteria in sputum samples from cystic fibrosis patients. The Nontuberculous Mycobacteria in Cystic Fibrosis Study Group.Journal of clinical microbiology · 1997Trial
- Performance of an automated Q-beta replicase amplification assay for Mycobacterium tuberculosis in a clinical trial.Journal of clinical microbiology · 1997Trial
- Fluorescent Carbon Dots from Disposable Masks: Pathogen Sensing and UV-Blocking Film Integration.Journal of fluorescence · 2025Article
- Article
- Metabolomic Profiling of Plasma from Patients with Tuberculosis by Use of Untargeted Mass Spectrometry Reveals Novel Biomarkers for Diagnosis.Journal of clinical microbiology · 2015Article
- Identification of specific metabolites in culture supernatant of Mycobacterium tuberculosis using metabolomics: exploration of potential biomarkers.Emerging microbes & infections · 2015Article
- Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum.The American journal of tropical medicine and hygiene · 2014Article
- Evaluation of in-house polymerase chain reaction assay sensitivity, can it be utilized in limited-resources settings?Medical journal of the Islamic Republic of Iran · 2014Article
- Rapid detection of the Mycobacterium tuberculosis complex by use of quenching probe PCR (geneCube).Journal of clinical microbiology · 2012Article
- Bayesian receiver operating characteristic estimation of multiple tests for diagnosis of bovine tuberculosis in Chadian cattle.PloS one · 2009Article
- Comparison of in-house PCR with conventional techniques and Cobas Amplicor M. tuberculosis kit for detection of Mycobacterium tuberculosis.Yonsei medical journal · 2008Article
- Review
- Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis.Journal of clinical microbiology · 2000Article
- Multicenter evaluation of the Abbott LCx Mycobacterium tuberculosis ligase chain reaction assay.Journal of clinical microbiology · 1999Article
- Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.Journal of clinical microbiology · 1999Article
- Clinical evaluation of the enhanced Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for rapid diagnosis of tuberculosis in prison inmates.Journal of clinical microbiology · 1999Article
- Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in human samples.Journal of clinical microbiology · 1999Article
- Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and nonrespiratory specimens.Journal of clinical microbiology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sputum specimens received for the diagnosis of tuberculosis or other mycobacterial infections were tested by a PCR-based assay and culture techniques. Results of the PCR assay (Amplicor Mycobacterium tuberculosis Test) were compared with results of standard culture techniques with cultures held for 6 weeks. One thousand nine specimens were included: 301 retrospective specimens (frozen at -70 degrees C and later tested by PCR) and 708 prospective specimens (tested within 1 day of processing). One hundred sixty-two (16%) of the specimens were culture positive for M. tuberculosis; 83 (51%) of these were also fluorochrome stain positive. The sensitivity and specificity of the Amplicor PCR compared with those of culture were 83% (134 of 162 specimens) and 97% (800 of 827 specimens), respectively. The sensitivity for fluorochrome stain-positive specimens was 99%, and that for fluorochrome stain-negative specimens was 66%. The great majority of the 28 PCR-negative, culture-positive specimens were low positives; 27 were smear negative and 19 contained < 100 CFU of M. tuberculosis per ml. The 27 PCR-positive, culture-negative specimens included 24 that were positive by repeat testing by alternate primer PCR and were from patients with tuberculosis on antimicrobial therapy. With these considered culture misses, the final sensitivities of PCR and culture were 85, and 87%, respectively, while the specificities were 99.6 and 100%, respectively. After normal laboratory processing of sputum specimens, the Amplicor PCR assay can be completed in 8 h. Thus, it is possible to have results available within 10 h of specimen submission.
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