ArticleThe Journal of cell biology1996
Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 162 citations in OpenAlex.
- Measuring Molecular Mass Densities at Subcellular Resolution Using Optical Diffraction Tomography.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A checkpoint function for Nup98 in nuclear pore formation suggested by novel inhibitory nanobodies.The EMBO journal · 2024Article
- Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.Cold Spring Harbor perspectives in biology · 2022Review
- Exportins can inhibit major mitotic assembly eventsNucleus (Austin, Tex.) · 2020Article
- Cell-free transcription inThe Journal of biological chemistry · 2019Article
- Consequences of a tight squeeze: Nuclear envelope rupture and repair.Nucleus (Austin, Tex.) · 2017Review
- Epigenetic reprogramming of the zygote in mice and men: on your marks, get set, go!Reproduction (Cambridge, England) · 2016Review
- Nucleosome functions in spindle assembly and nuclear envelope formation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Cell biology: nuclear dilemma resolved.Nature · 2015Article
- Targeting of viral capsids to nuclear pores in a cell-free reconstitution system.Traffic (Copenhagen, Denmark) · 2014Article
- Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration.Molecular biology of the cell · 2014Article
- Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.Methods in cell biology · 2014Article
- Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly, and chromatin de-condensation.Chromosoma · 2012Review
- Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly.The Journal of cell biology · 2011Article
- Nuclear import of an intact preassembled proteasome particle.Molecular biology of the cell · 2011Article
- Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.Molecular biology of the cell · 2010Article
- Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.The Journal of biological chemistry · 2009Article
- Transportin mediates nuclear entry of DNA in vertebrate systems.Traffic (Copenhagen, Denmark) · 2009Article
- Importin beta regulates the seeding of chromatin with initiation sites for nuclear pore assembly.Molecular biology of the cell · 2009Article
- Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A key event in nuclear formation is the assembly of functional nuclear pores. We have used a nuclear reconstitution system derived from Xenopus eggs to examine the process of nuclear pore assembly in vitro. With this system, we have identified three reagents which interfere with nuclear pore assembly, NEM, GTP gamma S, and the Ca++ chelator, BAPTA. These reagents have allowed us to determine that the assembly of a nuclear pore requires the prior assembly of a double nuclear membrane. Inhibition of nuclear vesicle fusion by pretreatment of the membrane vesicle fraction with NEM blocks pore complex assembly. In contrast, NEM treatment of already fused double nuclear membranes does not block pore assembly. This indicates that NEM inhibits a single step in pore assembly--the initial fusion of vesicles required to form a double nuclear membrane. The presence of GTP gamma S blocks pore assembly at two distinct steps, first by preventing fusion between nuclear vesicles, and second by blocking a step in pore assembly that occurs on already fused double nuclear membranes. Interestingly, when the Ca2+ chelator BAPTA is added to a nuclear assembly reaction, it only transiently blocks nuclear vesicle fusion, but completely blocks nuclear pore assembly. This results in the formation of a nucleus surrounded by a double nuclear membrane, but devoid of nuclear pores. To order the positions at which GTP gamma S and BAPTA interfere with pore assembly, a novel anchored nuclear assembly assay was developed. This assay revealed that the BAPTA-sensitive step in pore assembly occurs after the second GTP gamma S-sensitive step. Thus, through use of an in vitro nuclear reconstitution system, it has been possible to biochemically define and order multiple steps in nuclear pore assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.