ArticleThe Journal of cell biology1996
Lysophosphatidic acid and bFGF control different modes in proliferating myoblasts.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 75 citations in OpenAlex.
- Grb2/Sos1 signaling regulates the number of reserve cells in C2C12 cell culture.In vitro cellular & developmental biology. Animal · 2026Article
- Single-cell transcriptome dynamics of the autotaxin-lysophosphatidic acid axis during muscle regeneration reveal proliferative effects in mesenchymal fibro-adipogenic progenitors.Frontiers in cell and developmental biology · 2023Article
- A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation.Nature food · 2022Article
- Phosphatidic Acid Stimulates Myoblast Proliferation through Interaction with LPA1 and LPA2 Receptors.International journal of molecular sciences · 2021Article
- Lysophosphatidic Acid Inhibits Simvastatin-Induced Myocytoxicity by Activating LPA Receptor/PKC Pathway.Molecules (Basel, Switzerland) · 2020Article
- Janus effect of glucocorticoids on differentiation of muscle fibro/adipogenic progenitors.Scientific reports · 2020Article
- CD4 T-cells regulate angiogenesis and myogenesis.Biomaterials · 2018Article
- Group I Paks support muscle regeneration and counteract cancer-associated muscle atrophy.Journal of cachexia, sarcopenia and muscle · 2018Article
- A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility.Molecular and cellular biology · 2016Article
- CCN family protein 2 (CCN2) promotes the early differentiation, but inhibits the terminal differentiation of skeletal myoblasts.Journal of biochemistry · 2015Article
- Bioactive lipids, LPC and LPA, are novel prometastatic factors and their tissue levels increase in response to radio/chemotherapy.Molecular cancer research : MCR · 2014Article
- Silencing of drpr leads to muscle and brain degeneration in adult Drosophila.The American journal of pathology · 2014Article
- Strategies to improve regeneration of the soft palate muscles after cleft palate repair.Tissue engineering. Part B, Reviews · 2012Review
- Article
- Human muscle satellite cells show age-related differential expression of S100B protein and RAGE.Age (Dordrecht, Netherlands) · 2011Article
- The myogenic kinome: protein kinases critical to mammalian skeletal myogenesis.Skeletal muscle · 2011Article
- Generation of a monoclonal antibody reactive to prefusion myocytes.Journal of muscle research and cell motility · 2011Article
- G-protein coupled receptors in stem cell self-renewal and differentiation.Stem cell reviews and reports · 2010Review
- Dual roles of smad proteins in the conversion from myoblasts to osteoblastic cells by bone morphogenetic proteins.The Journal of biological chemistry · 2010Article
- Mechanisms of the lysophosphatidic acid-induced increase in [Ca(2+)](i) in skeletal muscle cells.Journal of cellular and molecular medicine · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myogenic cells provide excellent in vitro models for studying the cell growth and differentiation. In this study we report that lysophosphatidic acid (LPA), a bioactive phospholipid contained in serum, stimulates the growth and inhibits the differentiation of mouse C2C12 myoblast cells, in a distinct manner from basic fibroblast growth factor (bFGF) whose mitotic and anti-differentiation actions have been well investigated. These actions of LPA were both blocked by pertussis toxin, suggesting the involvement of Gi class of G proteins, whereas bFGF acts through receptor tyrosine kinases. Detailed analysis revealed that LPA and bFGF act differently in regulating the myogenic basic helix-loop-helix (bHLH) proteins, the key players in myogenic differentiation process. LPA stimulates the proliferation of undifferentiated myoblasts allowing the continued expression of MyoD, but in contrast, bFGF does so with the MyoD expression suppressed at the mRNA level. Both compounds maintain the myf-5 expression, and suppress the myogenin expression. In addition, while LPA did not inhibit cell-cell contact-induced differentiation, bFGF strongly inhibited this process. Furthermore, LPA and bFGF act cooperatively in their mitogenic and anti-differentiation abilities. These findings indicate that LPA and bFGF differently stimulate intracellular signaling pathways, resulting in proliferating myoblasts each bearing a distinct expression pattern of myogenic bHLH proteins and distinct differentiation potentials in response to cell-cell contact, and illustrate the biological significance of Gi-mediated and tyrosine kinase-mediated signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.