ArticleThe Journal of membrane biology1995
Human macrophages contain a stretch-sensitive potassium channel that is activated by adherence and cytokines.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 57 citations in OpenAlex.
- Targeting myeloid cell-mediated fibrosis through FAK.Nature biomedical engineering · 2026Article
- ICI-induced cardiovascular toxicity: mechanisms and immune reprogramming therapeutic strategies.Frontiers in immunology · 2025Review
- Protective effect of low-intensity pulsed ultrasound on immune checkpoint inhibitor-related myocarditis via fine-tuning CD4Cancer immunology, immunotherapy : CII · 2024Article
- Downregulation of calponin 2 contributes to the quiescence of lung macrophages.American journal of physiology. Cell physiology · 2019Article
- Physical and mechanical regulation of macrophage phenotype and function.Cellular and molecular life sciences : CMLS · 2015Review
- Immune mediators of postoperative ileus.Langenbeck's archives of surgery · 2012Review
- A potassium ion channel is involved in cytokine production by activated human macrophages.Clinical and experimental immunology · 2002Article
- Mechanosensitive cation channels in human leukaemia cells: calcium permeation and blocking effect.The Journal of physiology · 2002Article
- Article
- Biomechanical regulation of human monocyte/macrophage molecular function.The American journal of pathology · 2000Article
- HIV-1 gp120 and chemokines activate ion channels in primary macrophages through CCR5 and CXCR4 stimulation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Macrophages from inflamed but not normal glomeruli are unresponsive to anti-inflammatory cytokines.The American journal of pathology · 2000Article
- Involvement of stretch-activated Cl- channels in ramification of murine microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A variety of stimuli, including cytokines and adhesion to surfaces and matrix proteins, can regulate macrophage function, in part through changes in Ca(2+)- dependent second messengers. While fluctuation in intracellular Ca2+ is an important modulator of cellular activation, little attention has been paid to the roles of other ions whose cytoplasmic concentrations can be rapidly regulated by ion channels. To examine the role of ion channels in macrophage function, we undertook patch clamp studies of human culture-derived macrophages grown under serum-free conditions. The major ionic current in these cells was carried by an outwardly rectifying K+ channel, which had a single-channel conductance of 229 pS in symmetrical K(+)-rich solution and macroscopic whole-cell conductance of 9.8 nS. These channels opened infrequently in resting cells but were activated immediately by (i) adhesion of mobile cells onto a substrate, (ii) stretch applied to isolated membrane patches in Ca(2+)-free buffers, (iii) intracellular Ca2+ (EC50 of 0.4 microM), and (iv) the cytokine IL-2. Furthermore, barium and 4-aminopyridine, blockers of this channel, altered the organization and structure of the cytoskeletal proteins actin, tubulin and vimentin. These cytoskeletal changes were associated with reversible alteration to the morphology of the cells. Thus, we have identified an outwardly rectifying K+ channel that appeared to be involved in cytokine and adherence-mediated macrophage activation, and in the maintenance of cytoskeletal integrity and cell shape.
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