ArticleInfection and immunity1996
Complete development of Cryptosporidium parvum in bovine fallopian tube epithelial cells.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 69 citations in OpenAlex.
- Use of nanoparticles, a modern means of drug delivery, against cryptosporidiosis.Journal of advanced veterinary and animal research · 2023Review
- In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds.Parasitology research · 2012Article
- Cryptosporidiosis-an overview.Journal of biomedical research · 2012Article
- Cell sorting-assisted microarray profiling of host cell response to Cryptosporidium parvum infection.Infection and immunity · 2010Article
- Cryptosporidium parvum DNA replication in cell-free culture.The Journal of parasitology · 2009Article
- Effect of select medium supplements on in vitro development of Cryptosporidium andersoni in HCT-8 cells.Parasitology research · 2009Article
- In vitro cultivation of cryptosporidium species.Clinical microbiology reviews · 2002Review
- Characterization of an intestinal epithelial cell receptor recognized by the Cryptosporidium parvum sporozoite ligand CSL.Infection and immunity · 2001Article
- A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts.Applied and environmental microbiology · 1999Article
- A role for host phosphoinositide 3-kinase and cytoskeletal remodeling during Cryptosporidium parvum infection.Infection and immunity · 1999Article
- An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture.Applied and environmental microbiology · 1997Article
- Synergistic anticryptosporidial potential of the combination alpha-1-antitrypsin and paromomycin.Antimicrobial agents and chemotherapy · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidium parvum is a coccidian parasite responsible for causing protracted and life-threatening diarrheal illness in immunocompromised humans, especially patients with AIDS. The lack of medications effective in treating people suffering from cryptosporidiosis has prompted the development of in vivo and in vitro models for this disease. This study is the first to demonstrate that C. parvum can complete its entire life cycle (from sporozoite to infective oocyst) in a primary culture of bovine fallopian tube epithelial (BFTE) cells. Scanning and transmission electron photomicrographs were used to detail the ultrastructure of individual parasitic stages. Successful infections were produced by inoculating cell cultures with either oocysts or purified sporozoites. Infection of BFTE cells with C. parvum close paralleled in vivo infections with regard to host cell location and chronology of parasite development. Infecting BFTE cells with sporulated oocysts provided a reproducible and quantitative cultivation system with significantly (P < or = 0.001) higher infection rates than in Madin-Darby canine kidney cells. Oocysts produced in BFTE cells were infective for immunosuppressed adult C57BL/6N mice. Cultivation of C. parvum in BFTE cells will facilitate the study of interactions between parasites and host cells as well as provide a reliable system for evaluating anticryptosporidial compound efficacy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.