ArticleThe EMBO journal1995
Genetic analysis of an ARS element from the fission yeast Schizosaccharomyces pombe.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 135 citations in OpenAlex.
- Various short autonomously replicating sequences from the yeastCurrent research in microbial sciences · 2021Article
- Bioinformatical dissection of fission yeast DNA replication origins.Open biology · 2020Article
- Dynamics of DNA replication in a eukaryotic cell.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Chromosomal context and replication properties of ARS plasmids in Schizosaccharomyces pombe.Journal of biosciences · 2015Article
- Recent advances in the genome-wide study of DNA replication origins in yeast.Frontiers in microbiology · 2015Article
- Article
- Chromatin architectures at fission yeast transcriptional promoters and replication origins.Nucleic acids research · 2012Article
- Initiation of DNA Replication in the Human Genome.Hereditary genetics : current research · 2012Article
- OriDB, the DNA replication origin database updated and extended.Nucleic acids research · 2012Article
- Defining the replication program through the chromatin landscape.Critical reviews in biochemistry and molecular biology · 2011Review
- Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.The EMBO journal · 2010Article
- Genomic study of replication initiation in human chromosomes reveals the influence of transcription regulation and chromatin structure on origin selection.Molecular biology of the cell · 2010Article
- Why are we where we are? Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Molecular analysis of the replication program in unicellular model organisms.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Multiple mechanisms contribute to Schizosaccharomyces pombe origin recognition complex-DNA interactions.The Journal of biological chemistry · 2008Article
- Checkpoint independence of most DNA replication origins in fission yeast.BMC molecular biology · 2007Article
- Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast.The EMBO journal · 2007Article
- DNA elements modulating the KARS12 chromosomal replicator in Kluyveromyces lactis.Molecular genetics and genomics : MGG · 2007Article
- Atomic force microscopy of DNA in solution and DNA modelling show that structural properties specify the eukaryotic replication initiation site.Nucleic acids research · 2007Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
ARS (autonomously replicating sequence) elements are DNA fragments that can function as origins of DNA replication in yeast. We report the first fine-structure analysis of ars1, an ARS element of the fission yeast Schizosaccharomyces pombe. Characterization of a series of nested deletion mutations indicated that the minimal fragment of DNA encompassing ars1 is surprisingly large. No fragment < 650 bp retained significant ARS activity. Analysis of deletion and substitution mutations scanning the entire minimal ars1 identified a single essential 50 bp fragment (segment 1). Only one other 50 bp mutation reduced activity as much as 5-fold and most deletions were without effect. Thus, the minimal ars1 is composed of two general types of genetic elements, a small segment that is absolutely required for efficient ARS activity and a much larger region that is tolerant of internal structural alterations. Higher resolution analysis of segment 1 defined a critical 30 bp A/T-rich segment which appears to contain redundant genetic elements. Schizosaccharomyces pombe ars1 promoted high frequency transformation in the budding yeast S.cerevisiae but this heterologous activity was not dependent on segment 1. Our analysis indicates that the functional elements required for ARS function in S.pombe and S.cerevisiae are clearly different.
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