ArticleThe EMBO journal1995
Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 115 citations in OpenAlex.
- Rice RS2-9, which is bound by transcription factor OSH1, blocks enhancer-promoter interactions in plants.The Plant journal : for cell and molecular biology · 2022Article
- Spermiogenesis and Male Fertility Require the Function of Suppressor of Hairy-Wing in Somatic Cyst Cells ofGenetics · 2018Article
- Article
- Highly conserved ENY2/Sus1 protein binds to Drosophila CTCF and is required for barrier activity.Epigenetics · 2014Article
- MOD(MDG4)-64.2 protein, isoform of MOD(MDG4) loci, directly interacts with the Tweedle protein family of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2013Article
- Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators.Plant cell reports · 2012Review
- Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex.Molecular genetics and genomics : MGG · 2011Article
- Identification of functional elements and regulatory circuits by Drosophila modENCODE.Science (New York, N.Y.) · 2010Article
- E(y)2/Sus1 is required for blocking PRE silencing by the Wari insulator in Drosophila melanogaster.Chromosoma · 2010Article
- Chromatin insulator and the promoter targeting sequence modulate the timing of long-range enhancer-promoter interactions in the Drosophila embryo.Developmental biology · 2010Article
- Drosophila mini-white model system: new insights into positive position effects and the role of transcriptional terminators and gypsy insulator in transgene shielding.Nucleic acids research · 2010Article
- Analysis of chromatin boundary activity in Drosophila cells.BMC molecular biology · 2008Article
- Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements.Nucleic acids research · 2008Article
- Enhancer-promoter communication is regulated by insulator pairing in a Drosophila model bigenic locus.Molecular and cellular biology · 2008Article
- Context differences reveal insulator and activator functions of a Su(Hw) binding region.PLoS genetics · 2008Article
- Article
- Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs.Nucleic acids research · 2008Article
- New properties of Drosophila fab-7 insulator.Genetics · 2007Article
- Analysis of the H19ICR insulator.Molecular and cellular biology · 2007Article
- A genetic screen supports a broad role for the Drosophila insulator proteins BEAF-32A and BEAF-32B in maintaining patterns of gene expression.Molecular genetics and genomics : MGG · 2007Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The suppressor of Hairy-wing [su(Hw)] protein mediates the mutagenic effects of the gypsy retrotransposon by blocking enhancer activity. These repressive effects are general, can occur over long distances and require that the su(Hw) protein is bound between the affected enhancer and promoter. The effects of the su(Hw) binding region on yolk protein (yp) gene expression were determined. These genes are regulated by shared enhancers in the intergenic region, which provided a method to examine whether an enhancer blocked by the su(Hw) protein remained functional. We demonstrate that a blocked enhancer is completely active, supporting the proposal that the su(Hw) protein is an insulator protein that acts by forming a new boundary in a pre-existing chromatin domain, thereby preventing the interaction of regulatory elements located upstream of the insertion site with the promoter. In addition, we found that yp promoter function is not diminished by sharing enhancers, suggesting that these enhancers are not rate limiting for transcriptional activation. Lastly, our data indicate that yp promoter activity is interdependent, such that transcription from one promoter influences the level of activity of the linked promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.