ArticleThe EMBO journal1995
Identification of a cdk-activating kinase in fission yeast.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 89 citations in OpenAlex.
- Cimicifugin, a broad-spectrum inhibitor ofAntimicrobial agents and chemotherapy · 2024Article
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Immortalization of primary cells derived from the endangered Ryukyu long-furred rat.In vitro cellular & developmental biology. Animal · 2023Article
- CDK Regulation of Meiosis: Lessons fromGenes · 2020Review
- Cdk7 mediates RPB1-driven mRNA synthesis in Toxoplasma gondii.Scientific reports · 2016Article
- Article
- Functional evolution of cyclin-dependent kinases.Molecular biotechnology · 2009Review
- p27Kip1 inhibits cyclin D-cyclin-dependent kinase 4 by two independent modes.Molecular and cellular biology · 2009Article
- Identification of RIP1 kinase as a specific cellular target of necrostatins.Nature chemical biology · 2008Article
- Article
- Potential roles for autophosphorylation, kinase activity, and abundance of a CDK-activating kinase (Ee;CDKF;1) during growth in leafy spurge.Plant molecular biology · 2007Article
- Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo.Molecular and cellular biology · 2006Article
- Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.Molecular biology of the cell · 2005Article
- The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis.The Plant cell · 2004Article
- cdk-7 Is required for mRNA transcription and cell cycle progression in Caenorhabditis elegans embryos.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The rice cyclin-dependent kinase-activating kinase R2 regulates S-phase progression.The Plant cell · 2002Article
- Specificity of Cdk activation in vivo by the two Caks Mcs6 and Csk1 in fission yeast.The EMBO journal · 2001Article
- A role for the START gene-specific transcription factor complex in the inactivation of cyclin B and Cut2 destruction.Molecular biology of the cell · 2000Article
- A pcl-like cyclin activates the Res2p-Cdc10p cell cycle "start" transcriptional factor complex in fission yeast.Molecular biology of the cell · 2000Article
- Regulation of cyclin-dependent kinases in Arabidopsis thaliana.Plant molecular biology · 2000Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have identified a second cyclin-dependent kinase (cdk) in fission yeast, crk1, which encodes a 335 amino acid protein that is most closely related to the KIN28 gene product from Saccharomyces cerevisiae and to a cdk activating kinase (CAK) encoded by the MO15 gene from Xenopus laevis, crk1 is essential for viability and delta crk1 cells arrest with septa and condensed chromatin. We show that Crk1 associates with the Mcs2 mitotic catastrophe suppressor, a cyclin H-like molecule, and overexpression of crk1 rescues the cell-cycle arrest defect of a mcs2-75 cdc2-3w cdc25-22 triple mutant at high temperature. The Crk1-Mcs2 complex possesses CAK activity in vitro in that it phosphorylates human Cdk2 on Thr160 which results in its activation in the presence of cyclin A. In addition Crk1-Mcs2 effectively phosphorylates a peptide corresponding to the C-terminal repeat domain (CTD) of RNA polymerase II. We demonstrate that crk1 is allelic to the mcs6 mitotic catastrophe suppressor and that the X.laevis MO15 gene rescues the cell-cycle arrest of an mcs6-13 cdc2-3w cdc25-22 at high temperature. Together these data suggest that the Crk1-Mcs2 complex is a CAK that interacts genetically with Cdc2 in fission yeast.
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Registered trials
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