ArticleProceedings of the National Academy of Sciences of the United States of America1996
Receptor-mediated gene transfer into macrophages.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 175 citations in OpenAlex.
- Oversized liposomes boost macrophage-targeted RNA delivery to regulate macrophage polarity.Molecular therapy. Nucleic acids · 2023Article
- Mannosylated Cationic Copolymers for Gene Delivery to Macrophages.Macromolecular bioscience · 2021Article
- Self-amplifying RNA vaccines for infectious diseases.Gene therapy · 2021Review
- Maximizing gene delivery efficiencies of cationic helical polypeptides via balanced membrane penetration and cellular targeting.Biomaterials · 2014Article
- Development of non-viral vehicles for targeted gene transfer into microglia via the integrin receptor CD11b.Frontiers in molecular neuroscience · 2014Article
- Mannosylated chitosan nanoparticles for delivery of antisense oligonucleotides for macrophage targeting.BioMed research international · 2014Article
- An agent-based model of the response to angioplasty and bare-metal stent deployment in an atherosclerotic blood vessel.PloS one · 2014Article
- Cationic, helical polypeptide-based gene delivery for IMR-90 fibroblasts and human embryonic stem cells.Biomaterials science · 2013Article
- Reactive and bioactive cationic α-helical polypeptide template for nonviral gene delivery.Angewandte Chemie (International ed. in English) · 2012Article
- Advances in Systemic siRNA Delivery.Drugs of the future · 2009Article
- Polymeric Materials for Gene Delivery and DNA Vaccination.Advanced materials (Deerfield Beach, Fla.) · 2009Article
- Respiratory syncytial virus infection: from biology to therapy: a perspective.The World Allergy Organization journal · 2008Article
- Needle-like morphology of H2K4b polyplexes associated with increases in transfection in vitro.Cancer therapy · 2007Article
- Mannan-mediated gene delivery for cancer immunotherapy.Immunology · 2007Article
- Design and development of polymers for gene delivery.Nature reviews. Drug discovery · 2005Review
- Protecting from R5-tropic HIV: individual and combined effectiveness of a hammerhead ribozyme and a single-chain Fv antibody that targets CCR5.Gene therapy · 2004Article
- Gene therapy of cystic fibrosis (CF) airways: a review emphasizing targeting with lactose.Glycoconjugate journal · 2001Review
- Dendritic cell delivery of plasmid DNA. Applications for controlled genetic immunization.Molecular biotechnology · 2001Review
- Enhancement of MSH receptor- and GAL4-mediated gene transfer by switching the nuclear import pathway.Gene therapy · 2001Article
- Supramolecular structure and nuclear targeting efficiency determine the enhancement of transfection by modified polylysines.Gene therapy · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Gene transfer systems targeting various receptors have been developed to introduce functional genes into cells in culture and into intact animals. A synthetic molecular conjugate, consisting of mannosylated polylysine that exploits endocytosis via the macrophage mannose receptor, was constructed and complexed to expression plasmids containing either the Photinus pyralis luciferase or Escherichia coli beta-galactosidase (lacZ) reporter genes. The DNA complexes were used to transfect murine macrophages isolated from peritoneal exudates in vitro. Luciferase and beta-galactosidase activity was found in transfected cells in culture, whereas complexes consisting of an irrelevant plasmid bound to mannosylated polylysine or the expression plasmid bound to galactosylated polylysine resulted in no detectable transgene expression. Gene transfer was inhibited by the addition of excess mannosylated bovine serum albumin to the culture medium before transfection. Reporter genes were also transferred into macrophages residing in the spleen and liver of adult animals using this system. Luciferase activity was maximal at 4 days after transfection and decreased to lower levels by 16 days. Transgene expression conformed to the distribution of cells that had nonspecific esterase, a cytochemical marker for macrophages. Thus, this system can be used to introduce functional genes into macrophages and may be an approach to the treatment of storage diseases that affect the reticuloendothelial system.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.