ArticleMolecular and cellular biology1996
E2F-1 blocks terminal differentiation and causes proliferation in transgenic megakaryocytes.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- Unraveling the impact of crizotinib to promote megakaryopoiesis for alleviating thrombocytopenia in myelodysplastic neoplasms.Leukemia · 2025Article
- Fusion oncoproteins and cooperating mutations define disease phenotypes in NUP98-rearranged leukemia.Blood · 2025Article
- Whole genome sequence analysis of platelet traits in the NHLBI Trans-Omics for Precision Medicine (TOPMed) initiative.Human molecular genetics · 2022Article
- E2F1 impairs all-trans retinoic acid-induced osteogenic differentiation of osteosarcoma via promoting ubiquitination-mediated degradation of RARα.Cell cycle (Georgetown, Tex.) · 2014Article
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- Developmental stage-specific interplay of GATA1 and IGF signaling in fetal megakaryopoiesis and leukemogenesis.Genes & development · 2010Article
- Emerging roles of E2Fs in cancer: an exit from cell cycle control.Nature reviews. Cancer · 2009Review
- TRIP-Br2 promotes oncogenesis in nude mice and is frequently overexpressed in multiple human tumors.Journal of translational medicine · 2009Article
- E2F6 inhibits cobalt chloride-mimetic hypoxia-induced apoptosis through E2F1.Molecular biology of the cell · 2008Article
- Proapoptotic role of novel gene-expression factors.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2007Review
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- The adenovirus E4-6/7 protein directs nuclear localization of E2F-4 via an arginine-rich motif.Journal of virology · 2005Article
- Critical role of active repression by E2F and Rb proteins in endoreplication during Drosophila development.The EMBO journal · 2003Article
- Constitutive E2F1 overexpression delays endochondral bone formation by inhibiting chondrocyte differentiation.Molecular and cellular biology · 2003Article
- Cell cycle proteins exhibit altered expression patterns in lentiviral-associated encephalitis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor.The EMBO journal · 2002Article
- The p107 tumor suppressor induces stable E2F DNA binding to repress target promoters.Oncogene · 2001Article
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- Induction of cell-cycle regulators in simian immunodeficiency virus encephalitis.The American journal of pathology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription factor E2F-1 plays a central role in the cell cycle through its ability to activate genes involved in cell division. E2F-1 activity is regulated by a number of proteins, including the retinoblastoma susceptibility gene product, cyclin-dependent kinases, and their inhibitors, proteins that have been implicated in the control of certain developmental processes. To investigate a potential role of E2F-1 in differentiation, we assayed the ability of megakaryocytes to form platelets in an in vivo transgenic model. E2F-1 expression in megakaryocytes blocked differentiation during maturation, resulting in severe thrombocytopenia. Ultrastructural analysis of megakaryocytes revealed abnormal development characterized by hyperdemarcation of cytoplasmic membranes and reduced numbers of alpha granules. Administration of megakaryocyte growth and development factor or interleukin 6 could not overcome the differentiation block. Additionally, E2F-1 caused massive megakaryocyte accumulation in both normal and ectopic sites, first evident in E15 embryonic liver. Furthermore, significant apoptosis was observed in transgenic megakaryocytes. These data indicate that E2F-1 can prevent terminal differentiation, probably through its cell cycle-stimulatory activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.