ArticleMolecular and cellular biology1996
The major histocompatibility complex class II Ea promoter requires TFIID binding to an initiator sequence.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions.Analytical biochemistry · 2016Article
- Lineage-specific and ubiquitous biological roles of the mammalian transcription factor LSF.Gene · 2004Review
- Major histocompatibility complex class II transcriptional platform: assembly of nuclear factor Y and regulatory factor X (RFX) on DNA requires RFX5 dimers.Molecular and cellular biology · 2002Article
- Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex.Molecular and cellular biology · 1998Article
- Transcription of the juvenile hormone esterase gene under the control of both an initiator and AT-rich motif.The Biochemical journal · 1998Article
- Role of the transcription start site core region and transcription factor YY1 in Rous sarcoma virus long terminal repeat promoter activity.Journal of virology · 1998Article
- A survey of 178 NF-Y binding CCAAT boxes.Nucleic acids research · 1998Review
- Mechanism of synergy between TATA and initiator: synergistic binding of TFIID following a putative TFIIA-induced isomerization.Genes & development · 1997Article
- A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues.Nucleic acids research · 1997Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The major histocompatibility complex (MHC) class II Ea promoter is dependent on the presence of conserved upstream X and Y boxes and of initiator (Inr) sequences. In vitro transcription analysis of the Inr region with linker-scanning mutants pinpoints a functionally essential element that shows homology to the terminal deoxynucleotidyltransferase (TdT) Inr; contrary to the TdT Inr and other Inrs identified so far, the key sequence, between positions +5 and +12, is located within a transcribed area. Swapping the TdT sequence into the corresponding Ea position leads to a fivefold increase in transcription rate, without altering start site selection. Inr-binding proteins LBP-1/CP2 and TIP--a TdT Inr-binding protein unrelated to YY1--recognize the Ea Inr; they interact with overlapping yet distinct sequences around the Cap site, but their binding does not coincide with Ea Inr activity. A good correlation is, rather, found with binding of immunopurified holo-TFIID to this element. TFIID interacts both with Ea TATA-like and Inr sequences, but only the latter is functionally relevant. Unlike TBP, TFIID binds in the absence of TFIIA, indicating a stabilizing role for TBP-associated factors in Ea promoter recognition. Sequence comparison with other mouse and human MHC class II promoters suggests a common mechanism of start site(s) selection for the MHC class II gene family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.