ArticleMolecular & general genetics : MGG1995
Identification of a centromeric activity in the autonomously replicating TRA region allows improvement of the host-vector system for Candida maltosa.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe.Genome biology · 2015Article
- The evolutionary life cycle of the resilient centromere.Chromosoma · 2012Review
- How do microbial pathogens make CENs?PLoS pathogens · 2012Article
- Diversity in requirement of genetic and epigenetic factors for centromere function in fungi.Eukaryotic cell · 2011Review
- Inner kinetochore of the pathogenic yeast Candida glabrata.Eukaryotic cell · 2004Article
- The ribosomal RNA gene promoter and adjacent cis-acting DNA sequences govern plasmid DNA partitioning and stable inheritance in the parasitic protozoan Leishmania.Nucleic acids research · 2004Article
- Isolation and characterization of EPD1, an essential gene for pseudohyphal growth of a dimorphic yeast, Candida maltosa.Journal of bacteriology · 1998Article
- Evidence that part of a centromeric DNA region induces pseudohyphal growth in a dimorphic yeast, Candida maltosa.Journal of bacteriology · 1997Article
- An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica.Molecular and cellular biology · 1997Article
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6 authors.
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Abstract
A centromeric activity was identified in the previously isolated 3.8 kb DNA fragment that carries an autonomously replicating sequence (ARS) from the yeast Candida maltosa. Plasmids bearing duplicated copies of the centromeric DNA (dicentric plasmids) were physically unstable and structural rearrangements of the dicentric plasmids occurred frequently in the transformed cells. The centromeric DNA activity was dissociated from the ARS, which is 0.2 kb in size, and was delimited to a fragment at least 325 bp in length. The centromeric DNA region included the consensus sequences of CDEI (centromeric DNA element I) and an AT-rich CDEII-like region of Saccharomyces cerevisiae but had no homology to the functionally critical CDEIII consensus. A plasmid bearing the whole 3.8 kb fragment was present in 1-2 copies per cell and was maintained stably even under non-selective culture conditions, while a plasmid having only the 0.2 kb ARS was unstable and accumulated to high copy numbers. The high-copy-number plasmid allowed us to overexpress a gene to a high level, which had never been attained before, under the control of both constitutive and inducible promoters in C. maltosa.
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