ArticleJournal of virology1996
Analysis of a nuclear localization signal of simian virus 40 major capsid protein Vp1.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- Recent advances in discovery and functional analysis of the small proteins and microRNA expressed by polyomaviruses.Virology · 2025Review
- An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Assembly of Structurally Simple Icosahedral Viruses.Sub-cellular biochemistry · 2024Review
- An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single-cells.bioRxiv : the preprint server for biology · 2023Article
- Article
- Role of a nuclear localization signal on the minor capsid proteins VP2 and VP3 in BKPyV nuclear entry.Virology · 2015Article
- DNA virus replication compartments.Journal of virology · 2014Review
- Formation of covalently modified folding intermediates of simian virus 40 Vp1 in large T antigen-expressing cells.Journal of virology · 2013Article
- NTS-Polyplex: a potential nanocarrier for neurotrophic therapy of Parkinson's disease.Nanomedicine : nanotechnology, biology, and medicine · 2012Review
- Molecular biology, epidemiology, and pathogenesis of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain.Clinical microbiology reviews · 2012Review
- Article
- Progressive multifocal leukoencephalopathy and promyelocytic leukemia nuclear bodies: a review of clinical, neuropathological, and virological aspects of JC virus-induced demyelinating disease.Acta neuropathologica · 2010Review
- NT-polyplex: a new tool for therapeutic gene delivery to neuroblastoma tumors.Cancer gene therapy · 2009Article
- The Polyomaviridae: Contributions of virus structure to our understanding of virus receptors and infectious entry.Virology · 2009Review
- JC virus minor capsid proteins Vp2 and Vp3 are essential for virus propagation.Journal of virology · 2006Article
- Characterization of two novel polyomaviruses of birds by using multiply primed rolling-circle amplification of their genomes.Journal of virology · 2006Article
- Major and minor capsid proteins of human polyomavirus JC cooperatively accumulate to nuclear domain 10 for assembly into virions.Journal of virology · 2004Article
- Importin alpha/beta and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal.Molecular biology of the cell · 2004Article
- Nuclear localization of avian polyomavirus structural protein VP1 is a prerequisite for the formation of virus-like particles.Journal of virology · 2004Article
- Analysis of DNA-binding activity of the JC virus minor capsid protein VP2.Journal of neurovirology · 2003Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The nuclear localization signal of the major structural protein, Vp1, of simian virus 40 was further defined by mutagenesis. The targeting activity was examined in cells microinjected with SV-Vp1 variant viral DNAs bearing either an initiation codon mutation of the agnoprotein or mutations in the Vp1 coding sequence or microinjected with pSG5-Vp1 and pSG5-Vp1 mutant DNAs in which Vp1 or mutant Vp1 is expressed from simian virus 40 early promoter. The Vp1 nuclear localization signal functioned autonomously without agno-protein once the Vp1 protein was synthesized in the cytoplasm. The targeting activity was localized to the amino-terminal 19 residues. While replacement of cysteine 10 with glycine, alanine, or serine did not affect the activity, replacement of arginine 6 with glycine caused the cytoplasmic phenotype. When multiple mutations were introduced among residue 5, 6, 7, 16, 17, or 19, the targeting activity was found to reside in two clusters of basic residues, a cluster of lysine 5, arginine 6, and lysine 7 and a cluster of lysine 16, lysine 17, and lysine 19. The clusters are independently important for nuclear localization activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.