ArticleJournal of bacteriology1996
Fine tangled pili expressed by Haemophilus ducreyi are a novel class of pili.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 78 citations in OpenAlex.
- Identification of FtpA, a Dps-Like Protein Involved in Anti-Oxidative Stress and Virulence in Actinobacillus pleuropneumoniae.Journal of bacteriology · 2022Article
- Identification of putative adhesins of Actinobacillus suis and their homologues in other members of the family Pasteurellaceae.BMC research notes · 2015Article
- A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis.The Journal of biological chemistry · 2014Article
- Article
- Coevolution of aah: a dps-like gene with the host bacterium revealed by comparative genomic analysis.TheScientificWorldJournal · 2012Article
- Characterization of the CpxRA regulon in Haemophilus ducreyi.Infection and immunity · 2010Article
- Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd.Journal of bacteriology · 2007Article
- Haemophilus ducreyi Outer membrane determinants, including DsrA, define two clonal populations.Infection and immunity · 2005Article
- PnuC and the utilization of the nicotinamide riboside analog 3-aminopyridine in Haemophilus influenzae.Antimicrobial agents and chemotherapy · 2004Article
- Iron-oxo clusters biomineralizing on protein surfaces: structural analysis of Halobacterium salinarum DpsA in its low- and high-iron states.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Dps protects cells against multiple stresses during stationary phase.Journal of bacteriology · 2004Article
- Nicotinamide ribosyl uptake mutants in Haemophilus influenzae.Infection and immunity · 2003Article
- Development of a rapid immunodiagnostic test for Haemophilus ducreyi.Journal of clinical microbiology · 2002Article
- Haemophilus ducreyi requires the flp gene cluster for microcolony formation in vitro.Infection and immunity · 2002Article
- An iron-binding protein, Dpr, from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro.Journal of bacteriology · 2002Article
- Cloning, overexpression, purification, and immunobiology of an 85-kilodalton outer membrane protein from Haemophilus ducreyi.Infection and immunity · 2001Article
- Transcription of candidate virulence genes of Haemophilus ducreyi during infection of human volunteers.Infection and immunity · 2001Article
- Expression of peptidoglycan-associated lipoprotein is required for virulence in the human model of Haemophilus ducreyi infection.Infection and immunity · 2000Article
- Role of the dpr product in oxygen tolerance in Streptococcus mutans.Journal of bacteriology · 2000Article
- Cloning and characterization of the lipooligosaccharide galactosyltransferase II gene of Haemophilus ducreyi.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Haemophilus ducreyi synthesizes fine, tangled pili composed predominantly of a protein whose apparent molecular weight is 24,000 (24K). A hybridoma, 2D8, produced a monoclonal antibody (MAb) that bound to a 24K protein in H. ducreyi strains isolated from diverse geographic locations. A lambda gt11 H. ducreyi library was screened with MAb 2D8. A 3.5-kb chromosomal insert from one reactive plaque was amplified and ligated into the pCRII vector. The recombinant plasmid, designated pHD24, expressed a 24K protein in Escherichia coli INV alpha F that bound MAb 2D8. The coding sequence of the 24K gene was localized by exonuclease III digestion. The insert contained a 570-bp open reading frame, designated ftpA (fine, tangled pili). Translation of ftpA predicted a polypeptide with a molecular weight of 21.1K. The predicted N-terminal amino acid sequence of the polypeptide encoded by ftpA was identical to the N-terminal amino acid sequence of purified pilin and lacked a cleavable signal sequence. Primer extension analysis of ftpA confirmed the lack of a leader peptide. The predicted amino acid sequence lacked homology to known pilin sequences but shared homology with the sequences of E. coli Dps and Treponema pallidum antigen TpF1 or 4D, proteins which associate to form ordered rings. An isogenic pilin mutant, H. ducreyi 35000ftpA::mTn3(Cm), was constructed by shuttle mutagenesis and did not contain pili when examined by electron microscopy. We conclude that H. ducreyi synthesizes fine, tangled pili that are composed of a unique major subunit, which may be exported by a signal sequence independent mechanism.
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