ArticleThe Histochemical journal1995
Effects of fixation on the preservation of peroxisomal structures for immunofluorescence studies using HepG2 cells as a model system.
Article in The Histochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed.
- Immunolabeling for Detection of Endogenous and Overexpressed Peroxisomal Proteins in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The respiratory chain inhibitor rotenone affects peroxisomal dynamics via its microtubule-destabilising activity.Histochemistry and cell biology · 2017Article
- Postfixation detergent treatment liberates the membrane modelling protein Pex11β from peroxisomal membranes.Histochemistry and cell biology · 2012Article
- Mammalian peroxisomes and reactive oxygen species.Histochemistry and cell biology · 2004Review
- FRET microscopy demonstrates molecular association of non-specific lipid transfer protein (nsL-TP) with fatty acid oxidation enzymes in peroxisomes.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immunofluorescence technique has become an important tool for the investigation of peroxisomes in cell culture. We have used this method for the study of peroxisomes in the human hepatoblastoma cell line HepG2. A marked heterogeneity of peroxisomal forms was detected. Besides spherical (about 100 nm) and rod-shaped structures (about 300 nm) many elongated, undulating tubular forms (up to 5 microns) were found. Further observations indicate that the appearance of the peroxisomal forms in immunofluorescence is dependent on the fixation procedure used. Whereas the fixation with methanol-acetone (-20 degrees C) or ethanol results in a punctate pattern with spherical particles, the use of formaldehyde/Triton X-100 fixation shows well-preserved tubules and rods. These observations may be of special importance for studies on the biogenesis of peroxisomes.
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Registered trials
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