ArticlePlant physiology1995
Isolation of a polyubiquitin promoter and its expression in transgenic potato plants.
Article in Plant physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 114 citations in OpenAlex.
- Transcriptome-based Mining of the Constitutive Promoters for Tuning Gene Expression in Aspergillus oryzae.Journal of microbiology (Seoul, Korea) · 2023Article
- Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression.Planta · 2022Article
- Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato.Plants (Basel, Switzerland) · 2020Article
- Article
- Agrobacterium-mediated transformation of the ascomycete mushroom Morchella importuna using polyubiquitin and glyceraldehyde-3-phosphate dehydrogenase promoter-based binary vectors.World journal of microbiology & biotechnology · 2018Article
- Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions.Scientific reports · 2017Article
- Comparison of the impact of viral and plant-derived promoters regulating selectable marker gene on maize transformation and transgene expression.Plant cell reports · 2017Article
- Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants.Frontiers in plant science · 2016Article
- Truncated Ubiquitin 5' Regulatory Region from Erianthus arundinaceus Drives Enhanced Transgene Expression in Heterologous Systems.Molecular biotechnology · 2015Article
- Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas.Planta · 2015Article
- Article
- The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.Plant cell reports · 2015Article
- BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism.BMC genomics · 2014Article
- A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière).International journal of molecular sciences · 2013Article
- Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1.Transgenic research · 2012Article
- Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants.Plant cell reports · 2012Article
- Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation.BMC biotechnology · 2011Article
- High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline.BMC plant biology · 2010Article
- Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA.Plant cell reports · 2010Article
- Promoter diversity in multigene transformation.Plant molecular biology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A polyubiquitin clone (ubi7) was isolated from a potato (Solanum tuberosum) genomic library using a copy-specific probe from a stress-induced ubiquitin cDNA. The genomic clone contained a 569-bp intron immediately 5' to the initiation codon for the first ubiquitin-coding unit. Two chimeric beta-glucuronidase (GUS) fusion transgenes were introduced into potato. The first contained GUS fused to a 1156-bp promoter fragment containing only 5' flanking and 5' untranslated sequences from ubi7. The second transgene contained GUS translationally fused to the carboxy terminus of the first ubiquitin-coding unit and thus included the intron present in the 5' untranslated region of the polyubiquitin gene. Both ubi7-GUS transgenes were activated by wounding in tuber tissue and in leaves by application of exogenous methyl jasmonate. They were also expressed constitutively in the potato tuber peel (outer 1-2 mm). Both transgenes were actively expressed in mature leaves. Exceptionally high levels of expression were observed in senescent leaves. Transgenic clones containing the ubi7 intron and the first ubiquitin-coding unit showed GUS expression levels at least 10 times higher than clones containing GUS fused to the intronless promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.