ArticleCurrent genetics1995
Multiple roles of the cellulase CBHI in enhancing production of fusion antibodies by the filamentous fungus Trichoderma reesei.
Article in Current genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Identification of a Bidirectional Promoter fromJournal of fungi (Basel, Switzerland) · 2022Article
- Nanoscale Infrared Spectroscopy and Chemometrics Enable Detection of Intracellular Protein Distribution.Analytical chemistry · 2020Article
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Modulating endoplasmic reticulum-Golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus Aspergillus oryzae.Applied and environmental microbiology · 2015Article
- Construction of a cellulase hyper-expression system in Trichoderma reesei by promoter and enzyme engineering.Microbial cell factories · 2012Article
- Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.Microbial cell factories · 2008Article
- High-yield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure.Applied and environmental microbiology · 2003Article
- Filamentous fungi as production organisms for glycoproteins of bio-medical interest.Glycoconjugate journal · 1999Review
- Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels.Applied and environmental microbiology · 1997Article
- Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains.Applied and environmental microbiology · 1996Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of Fab antibody fragments in Trichoderma reesei can be increased over 50-fold by fusing the core-linker region of the T. reesei cellulase CBHI (cellobiohydrolase I) to the heavy Fd chain (Nyyssönen et al. 1993). This beneficial role of CBHI in antibody production has now been studied further by comparisons of T. reesei trains producing the light chain only. Fab or CBHI-Fab all of which exhibited identical light chain integration. The N-terminal fusion of CBHI to the heavy Fd chain not only aided secretion, as expected, but also increased the level of mRNA encoding the CBHI-heavy Fd chain, either by stabilizing the messenger or by enhancing transcription. The CBHI part appeared to facilitate secretion at least by aiding the passage through the endoplasmic reticulum, since processing of the signal peptide of the antibody chains seemed to be most efficient in the strain producing CBHI-Fab in contrast to the strains producing light chain or Fab fragment. Interestingly, CBHI core-linker protein, originating from the CBHI-heavy Fd chain, was found in large amounts in the culture medium. The cleavage resulting in this tailless CBHI occurred inside the cell. This suggests that, by omitting the heterologous tail, the secretion of the resulting CBHI core-linker protein is enhanced to a level comparable with secretion of the extracellular T. reesei proteins.
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